TY - JOUR
T1 - Minimal Synthetic Cells to Study Integrin-Mediated Adhesion
AU - Frohnmayer, Johannes P.
AU - Brüggemann, Dorothea
AU - Eberhard, Christian
AU - Neubauer, Stefanie
AU - Mollenhauer, Christine
AU - Boehm, Heike
AU - Kessler, Horst
AU - Geiger, Benjamin
AU - Spatz, Joachim P.
N1 - Publisher Copyright:
© 2015 The Authors. Published by Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - To shed light on cell-adhesion-related molecular pathways, synthetic cells offer the unique advantage of a well-controlled model system with reduced molecular complexity. Herein, we show that liposomes with the reconstituted platelet integrin αIIbβ3 as the adhesion-mediating transmembrane protein are a functional minimal cell model for studying cellular adhesion mechanisms in a defined environment. The interaction of these synthetic cells with various extracellular matrix proteins was analyzed using a quartz crystal microbalance with dissipation monitoring. The data indicated that integrin was functionally incorporated into the lipid vesicles, thus enabling integrin-specific adhesion of the engineered liposomes to fibrinogen- and fibronectin-functionalized surfaces. Then, we were able to initiate the detachment of integrin liposomes from these surfaces in the presence of the peptide GRGDSP, a process that is even faster with our newly synthesized peptide mimetic SN529, which specifically inhibits the integrin αIIbβ3.
AB - To shed light on cell-adhesion-related molecular pathways, synthetic cells offer the unique advantage of a well-controlled model system with reduced molecular complexity. Herein, we show that liposomes with the reconstituted platelet integrin αIIbβ3 as the adhesion-mediating transmembrane protein are a functional minimal cell model for studying cellular adhesion mechanisms in a defined environment. The interaction of these synthetic cells with various extracellular matrix proteins was analyzed using a quartz crystal microbalance with dissipation monitoring. The data indicated that integrin was functionally incorporated into the lipid vesicles, thus enabling integrin-specific adhesion of the engineered liposomes to fibrinogen- and fibronectin-functionalized surfaces. Then, we were able to initiate the detachment of integrin liposomes from these surfaces in the presence of the peptide GRGDSP, a process that is even faster with our newly synthesized peptide mimetic SN529, which specifically inhibits the integrin αIIbβ3.
KW - cell adhesion
KW - integrin
KW - liposomes
KW - peptide mimetics
KW - quartz crystal microbalance
UR - http://www.scopus.com/inward/record.url?scp=84943198563&partnerID=8YFLogxK
U2 - 10.1002/anie.201503184
DO - 10.1002/anie.201503184
M3 - Article
C2 - 26257266
AN - SCOPUS:84943198563
SN - 1433-7851
VL - 54
SP - 12472
EP - 12478
JO - Angewandte Chemie International Edition in English
JF - Angewandte Chemie International Edition in English
IS - 42
ER -