Minimal Synthetic Cells to Study Integrin-Mediated Adhesion

Johannes P. Frohnmayer, Dorothea Brüggemann, Christian Eberhard, Stefanie Neubauer, Christine Mollenhauer, Heike Boehm, Horst Kessler, Benjamin Geiger, Joachim P. Spatz

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

To shed light on cell-adhesion-related molecular pathways, synthetic cells offer the unique advantage of a well-controlled model system with reduced molecular complexity. Herein, we show that liposomes with the reconstituted platelet integrin αIIbβ3 as the adhesion-mediating transmembrane protein are a functional minimal cell model for studying cellular adhesion mechanisms in a defined environment. The interaction of these synthetic cells with various extracellular matrix proteins was analyzed using a quartz crystal microbalance with dissipation monitoring. The data indicated that integrin was functionally incorporated into the lipid vesicles, thus enabling integrin-specific adhesion of the engineered liposomes to fibrinogen- and fibronectin-functionalized surfaces. Then, we were able to initiate the detachment of integrin liposomes from these surfaces in the presence of the peptide GRGDSP, a process that is even faster with our newly synthesized peptide mimetic SN529, which specifically inhibits the integrin αIIbβ3.

Original languageEnglish
Pages (from-to)12472-12478
Number of pages7
JournalAngewandte Chemie International Edition in English
Volume54
Issue number42
DOIs
StatePublished - 1 Oct 2015
Externally publishedYes

Keywords

  • cell adhesion
  • integrin
  • liposomes
  • peptide mimetics
  • quartz crystal microbalance

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