Milliliter-scale stirred tank reactors for the cultivation of microorganisms

Ralf Hortsch, Dirk Weuster-Botz

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

41 Scopus citations

Abstract

This review focuses on recent developments in the field of miniaturized stirred tank bioreactors for application in high-throughput bioprocess development. Different reactor concepts and their potential for parallel bioprocess development are discussed. A detailed description of important engineering state variables, their measurement at small-scale and their implication for scale-up and scale-down of bioprocesses are given. Examples of two different parallel cultivations at small-scale are presented: one with Escherichia coli and the other one with the filamentous microorganism Streptomyces tendae. It is shown that results obtained in parallelized milliliter-scale stirred tank reactors can be scaled up to the laboratory- and/or pilot-scale in a highly reliable manner. This helps to reduce development times for bioprocesses significantly. Finally, directions for future research are presented.

Original languageEnglish
Title of host publicationAdvances in Applied Microbiology
PublisherAcademic Press Inc.
Pages61-82
Number of pages22
EditionC
DOIs
StatePublished - 2010

Publication series

NameAdvances in Applied Microbiology
NumberC
Volume73
ISSN (Print)0065-2164

Fingerprint

Dive into the research topics of 'Milliliter-scale stirred tank reactors for the cultivation of microorganisms'. Together they form a unique fingerprint.

Cite this