Milk-Derived Amadori Products in Feces of Formula-Fed Infants

Nina Sillner, Alesia Walker, Daniel Hemmler, Monika Bazanella, Silke S. Heinzmann, Dirk Haller, Philippe Schmitt-Kopplin

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15 Scopus citations


Food processing of infant formula alters chemical structures, including the formation of Maillard reaction products between proteins and sugars. We detected early Maillard reaction products, so-called Amadori products, in stool samples of formula-fed infants. In total, four Amadori products (N-deoxylactulosyllysine, N-deoxyfructosyllysine, N-deoxylactulosylleucylisoleucine, N-deoxyfructosylleucylisoleucine) were identified by a combination of complementary nontargeted and targeted metabolomics approaches. Chemical structures were confirmed by preparation and isolation of reference compounds, LC-MS/MS, and NMR. The leucylisoleucine Amadori compounds, which most likely originate from β-lactoglobulin, were excreted throughout the first year of life in feces of formula-fed infants but were absent in feces of breastfed infants. Despite high inter- and intraindividual differences of Amadori products in the infants' stool, solid food introduction resulted in a continuous decrease, proving infant formula as the major source of the excreted Amadori products.

Original languageEnglish
Pages (from-to)8061-8069
Number of pages9
JournalJournal of agricultural and food chemistry
Issue number28
StatePublished - 17 Jun 2019


  • Amadori products
  • Maillard reaction
  • fructosyllysine
  • infant formula
  • metabolomics
  • milk marker


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