MicroRNA-9 controls dendritic development by targeting REST

Sebastian A. Giusti; Annette M. Vogl; Marisa M. Brockmann; Claudia A. Vercelli; Martin L. Rein; Dietrich Trümbach; Wolfgang Wurst; Demian Cazalla; Valentin Stein; Jan M. Deussing; Damian Refojo

doi:10.7554/eLife.02755

MicroRNA-9 controls dendritic development by targeting REST

Sebastian A. Giusti, Annette M. Vogl, Marisa M. Brockmann, Claudia A. Vercelli, Martin L. Rein, Dietrich Trümbach, Wolfgang Wurst, Demian Cazalla, Valentin Stein, Jan M. Deussing, Damian Refojo

Max Planck Institute of Psychiatry

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.

Original language	English
Article number	e02755
Journal	eLife
Volume	3
DOIs	https://doi.org/10.7554/eLife.02755
State	Published - 2014
Externally published	Yes

Keywords

REST
dendrite development
miR-9
miR-9 reporter
miR-9 sponge
mouse
neuroscience

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10.7554/eLife.02755

Cite this

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title = "MicroRNA-9 controls dendritic development by targeting REST",

abstract = "MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth. ",

keywords = "REST, dendrite development, miR-9, miR-9 reporter, miR-9 sponge, mouse, neuroscience",

author = "Giusti, {Sebastian A.} and Vogl, {Annette M.} and Brockmann, {Marisa M.} and Vercelli, {Claudia A.} and Rein, {Martin L.} and Dietrich Tr{\"u}mbach and Wolfgang Wurst and Demian Cazalla and Valentin Stein and Deussing, {Jan M.} and Damian Refojo",

year = "2014",

doi = "10.7554/eLife.02755",

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T1 - MicroRNA-9 controls dendritic development by targeting REST

AU - Giusti, Sebastian A.

AU - Vogl, Annette M.

AU - Brockmann, Marisa M.

AU - Vercelli, Claudia A.

AU - Rein, Martin L.

AU - Trümbach, Dietrich

AU - Wurst, Wolfgang

AU - Cazalla, Demian

AU - Stein, Valentin

AU - Deussing, Jan M.

AU - Refojo, Damian

PY - 2014

Y1 - 2014

N2 - MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.

AB - MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.

KW - REST

KW - dendrite development

KW - miR-9

KW - miR-9 reporter

KW - miR-9 sponge

KW - mouse

KW - neuroscience

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U2 - 10.7554/eLife.02755

DO - 10.7554/eLife.02755

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SN - 2050-084X

VL - 3

JO - eLife

JF - eLife

M1 - e02755

ER -

MicroRNA-9 controls dendritic development by targeting REST

Abstract

Keywords

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