MicroRNA-155 expression is enhanced by T-cell receptor stimulation strength and correlates with improved tumor control in Melanoma

Amaia Martinez-Usatorre, Lorenzo F. Sempere, Santiago J. Carmona, Laura Carretero-Iglesia, Gwennaëlle Monnot, Daniel E. Speiser, Nathalie Rufer, Alena Donda, Dietmar Zehn, Camilla Jandus, Pedro Romero

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

microRNAs are short noncoding RNAs that regulate protein expression posttranscriptionally.We previously showed that miR-155 promotes effector CD8+ T-cell responses. However, little is known about the regulation of miR-155 expression. Here, we report that antigen affinity and dose determine miR-155 expression in CD8+ T cells. In B16 tumors expressing a low-affinity antigen ligand, tumorspecific infiltrating CD8+ T cells showed variable miR-155 expression, whereby high miR-155 expression was associated with more cytokine-producing cells and tumor control. Moreover, anti-PD-1 treatment led to both increased miR-155 expression and tumor control by specific CD8+ T cells. In addition, miR-155 overexpression enhanced exhausted CD8+ T-cell persistence in the LCMV cl13 chronic viral infection model. In agreement with these observations in mouse models, miR-155 expression in human effector memory CD8+ T cells positively correlated with their frequencies in tumor-infiltrated lymph nodes of melanoma patients. Low miR-155 target gene signature in tumors was associated with prolonged overall survival in melanoma patients. Altogether, these results raise the possibility that high miR-155 expression in CD8+ tumorinfiltrating T cells may be a surrogate marker of the relative potency of in situ antigen-specific CD8+ T-cell responses.

Original languageEnglish
Pages (from-to)1013-1024
Number of pages12
JournalCancer Immunology Research
Volume7
Issue number6
DOIs
StatePublished - Jun 2019

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