Microglial phagolysosome dysfunction and altered neural communication amplify phenotypic severity in Prader-Willi Syndrome with larger deletion

Felipe Correa-da-Silva, Jenny Carter, Xin Yuan Wang, Rui Sun, Ekta Pathak, José Manuel Monroy Kuhn, Sonja C. Schriever, Clarissa M. Maya-Monteiro, Han Jiao, Martin J. Kalsbeek, Pedro M.M. Moraes-Vieira, Johan J.P. Gille, Margje Sinnema, Constance T.R.M. Stumpel, Leopold M.G. Curfs, Dirk Jan Stenvers, Paul T. Pfluger, Dominik Lutter, Alberto M. Pereira, Andries KalsbeekEric Fliers, Dick F. Swaab, Lawrence Wilkinson, Yuanqing Gao, Chun Xia Yi

Research output: Contribution to journalArticlepeer-review

Abstract

Prader-Willi Syndrome (PWS) is a rare neurodevelopmental disorder of genetic etiology, characterized by paternal deletion of genes located at chromosome 15 in 70% of cases. Two distinct genetic subtypes of PWS deletions are characterized, where type I (PWS T1) carries four extra haploinsufficient genes compared to type II (PWS T2). PWS T1 individuals display more pronounced physiological and cognitive abnormalities than PWS T2, yet the exact neuropathological mechanisms behind these differences remain unclear. Our study employed postmortem hypothalamic tissues from PWS T1 and T2 individuals, conducting transcriptomic analyses and cell-specific protein profiling in white matter, neurons, and glial cells to unravel the cellular and molecular basis of phenotypic severity in PWS sub-genotypes. In PWS T1, key pathways for cell structure, integrity, and neuronal communication are notably diminished, while glymphatic system activity is heightened compared to PWS T2. The microglial defect in PWS T1 appears to stem from gene haploinsufficiency, as global and myeloid-specific Cyfip1 haploinsufficiency in murine models demonstrated. Our findings emphasize microglial phagolysosome dysfunction and altered neural communication as crucial contributors to the severity of PWS T1's phenotype.

Original languageEnglish
Pages (from-to)64
Number of pages1
JournalActa Neuropathologica
Volume147
Issue number1
DOIs
StatePublished - 31 Mar 2024
Externally publishedYes

Keywords

  • Fornix
  • Glymphatic system
  • Hypothalamus
  • Immunosurveillance
  • Microglia
  • Myelin
  • Oxytocin

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