@inbook{eac0924347704146b309c392f07e777f,
title = "Microfluidic Production of Porous Polymer Cell-Mimics Capable of Gene Expression",
abstract = "Engineering simple, artificial models of living cells allows synthetic biologists to study cellular functions under well-controlled conditions. Reconstituting multicellular behaviors with synthetic cell-mimics is still a challenge because it requires efficient communication between individual compartments in large populations. This chapter presents a microfluidic method to produce large quantities of cell-mimics with highly porous, stable, and chemically modifiable polymer membranes that can be programmed on demand with nucleus-like DNA-hydrogel compartments for gene expression. We describe expression of genes encoded in the hydrogel compartment and communication between neighboring cell-mimics through diffusive protein signals.",
keywords = "Artificial cells, Cell-free transcription and translation, Communication, DNA hydrogel, Double emulsions, Microfluidics, Synthetic multicellular systems",
author = "Imre Banlaki and Lehr, {Fran{\c c}ois Xavier} and Henrike Niederholtmeyer",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1998-8_15",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "237--255",
booktitle = "Methods in Molecular Biology",
}