TY - GEN
T1 - Microfluidic multiplexed multi-analyte immunosensing platform
AU - Kling, A.
AU - Dincer, C.
AU - Chatelle, C.
AU - Armbrecht, L.
AU - Kieninger, J.
AU - Weber, W.
AU - Urban, G.
N1 - Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - In this work we present an electrochemical microfluidic platform for the detection of multiple analytes. The developed system allows the simultaneous readout of up to four different enzyme-linked assays even in various formats. The applicability was shown with the antibiotic detection of tetracycline in different concentrations by means of a DNA-based assay on a single chip. With a sample-to-result time of 15 min, a sample and reagent volume of less than 1 μl and a detectable concentration of 6.25 ng ml-1, a very sensitive, low-cost and fast immunosensing platform was established.
AB - In this work we present an electrochemical microfluidic platform for the detection of multiple analytes. The developed system allows the simultaneous readout of up to four different enzyme-linked assays even in various formats. The applicability was shown with the antibiotic detection of tetracycline in different concentrations by means of a DNA-based assay on a single chip. With a sample-to-result time of 15 min, a sample and reagent volume of less than 1 μl and a detectable concentration of 6.25 ng ml-1, a very sensitive, low-cost and fast immunosensing platform was established.
KW - DNA-based antibiotic assay
KW - Dry-film photoresist
KW - Electrochemical biosensor
KW - Multi-analyte
KW - Tetracycline
UR - http://www.scopus.com/inward/record.url?scp=84983341533&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84983341533
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1625
EP - 1627
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -