Microelectronic sensor system for microphysiological application on living cells

W. H. Baumann; M. Lehmann; A. Schwinde; R. Ehret; M. Brischwein; B. Wolf

doi:10.1016/S0925-4005(99)00116-1

Microelectronic sensor system for microphysiological application on living cells

W. H. Baumann, M. Lehmann, A. Schwinde, R. Ehret, M. Brischwein, B. Wolf

University of Rostock

Research output: Contribution to journal › Article › peer-review

142 Scopus citations

Abstract

Living cells can be considered as complex biochemical plants. Biochemical and biophysical processes enable a cell to maintain itself, to grow, to reproduce and to communicate with the environment. Getting more information about the multifunctional cellular processing of input- and output-signals in different cellular plants is essential for basic research as well as for various fields of biomedical applications. For in-vitro investigations on living cells, the cellular environment differs from the native environment found in vivo. As a first approach for on-line monitoring of cellular reactions under well controlled experimental conditions we have developed the so called Cell Monitoring System (CMS). It allows parallel and non-invasive measurement of different parameters from cellular systems by the use of microsensors. Microelectronic sensors are the adequate choice for the non-invasive measurement of environmental-as well as in- and output parameters of cells. In this paper we present a measurement system with pH-sensitive ISFETs (ionsensitive fieldeffect transistors) for the measurement of extracellular pH-related signals on cells and tissues.

Original language	English
Pages (from-to)	77-89
Number of pages	13
Journal	Sensors and Actuators, B: Chemical
Volume	55
Issue number	1
DOIs	https://doi.org/10.1016/S0925-4005(99)00116-1
State	Published - 25 Apr 1999
Externally published	Yes

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title = "Microelectronic sensor system for microphysiological application on living cells",

abstract = "Living cells can be considered as complex biochemical plants. Biochemical and biophysical processes enable a cell to maintain itself, to grow, to reproduce and to communicate with the environment. Getting more information about the multifunctional cellular processing of input- and output-signals in different cellular plants is essential for basic research as well as for various fields of biomedical applications. For in-vitro investigations on living cells, the cellular environment differs from the native environment found in vivo. As a first approach for on-line monitoring of cellular reactions under well controlled experimental conditions we have developed the so called Cell Monitoring System (CMS). It allows parallel and non-invasive measurement of different parameters from cellular systems by the use of microsensors. Microelectronic sensors are the adequate choice for the non-invasive measurement of environmental-as well as in- and output parameters of cells. In this paper we present a measurement system with pH-sensitive ISFETs (ionsensitive fieldeffect transistors) for the measurement of extracellular pH-related signals on cells and tissues.",

author = "Baumann, {W. H.} and M. Lehmann and A. Schwinde and R. Ehret and M. Brischwein and B. Wolf",

note = "Funding Information: We would like to thank Mr Alchalabi for the pNa and pK measurements with the ISFETs and Mr Bitzenhofer as well as Mr Zander for their technical support. We are very gratefull to Mr Sieben, Mr Gahle, Mr Weber, Mr Ruf and Mr Igel (Micronas Intermetall GmbH, Freiburg) for their support preparing the ISFETs. Parts of this investigations were kindly supported by grants of the Land Baden W{\"u}rttemberg (1992–1997) and by the Bundesministerium f{\"u}r Bildung, Wissenschaft, Forschung und Technologie (BMBF), German Ministry of Science and Technology Project No. 0310856 (1996–1999). ",

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AU - Wolf, B.

N1 - Funding Information: We would like to thank Mr Alchalabi for the pNa and pK measurements with the ISFETs and Mr Bitzenhofer as well as Mr Zander for their technical support. We are very gratefull to Mr Sieben, Mr Gahle, Mr Weber, Mr Ruf and Mr Igel (Micronas Intermetall GmbH, Freiburg) for their support preparing the ISFETs. Parts of this investigations were kindly supported by grants of the Land Baden Württemberg (1992–1997) and by the Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie (BMBF), German Ministry of Science and Technology Project No. 0310856 (1996–1999).

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N2 - Living cells can be considered as complex biochemical plants. Biochemical and biophysical processes enable a cell to maintain itself, to grow, to reproduce and to communicate with the environment. Getting more information about the multifunctional cellular processing of input- and output-signals in different cellular plants is essential for basic research as well as for various fields of biomedical applications. For in-vitro investigations on living cells, the cellular environment differs from the native environment found in vivo. As a first approach for on-line monitoring of cellular reactions under well controlled experimental conditions we have developed the so called Cell Monitoring System (CMS). It allows parallel and non-invasive measurement of different parameters from cellular systems by the use of microsensors. Microelectronic sensors are the adequate choice for the non-invasive measurement of environmental-as well as in- and output parameters of cells. In this paper we present a measurement system with pH-sensitive ISFETs (ionsensitive fieldeffect transistors) for the measurement of extracellular pH-related signals on cells and tissues.

AB - Living cells can be considered as complex biochemical plants. Biochemical and biophysical processes enable a cell to maintain itself, to grow, to reproduce and to communicate with the environment. Getting more information about the multifunctional cellular processing of input- and output-signals in different cellular plants is essential for basic research as well as for various fields of biomedical applications. For in-vitro investigations on living cells, the cellular environment differs from the native environment found in vivo. As a first approach for on-line monitoring of cellular reactions under well controlled experimental conditions we have developed the so called Cell Monitoring System (CMS). It allows parallel and non-invasive measurement of different parameters from cellular systems by the use of microsensors. Microelectronic sensors are the adequate choice for the non-invasive measurement of environmental-as well as in- and output parameters of cells. In this paper we present a measurement system with pH-sensitive ISFETs (ionsensitive fieldeffect transistors) for the measurement of extracellular pH-related signals on cells and tissues.

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Microelectronic sensor system for microphysiological application on living cells

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