TY - JOUR
T1 - Microbial homoserine lactones (AHLs) are effectors of root morphological changes in barley
AU - Rankl, Simone
AU - Gunsé, Benet
AU - Sieper, Tina
AU - Schmid, Christoph
AU - Poschenrieder, Charlotte
AU - Schröder, Peter
N1 - Publisher Copyright:
© 2016 Elsevier Ireland Ltd
PY - 2016/12/1
Y1 - 2016/12/1
N2 - While colonizing the rhizosphere, bacterial intra- and inter-specific communication is accomplished by N-Acyl-homoserine-lactones (AHLs) in a density-dependent manner. Moreover, plants are naturally exposed to AHLs and respond with tissue-specificity. In the present study, we investigated the influence of N-hexanoyl- (C6-HSL), N-octanoyl- (C8-HSL) and N-dodecanoyl-D/L-homoserine lactone (C12-HSL) on growth and root development in barley (Hordeum vulgare L.), and identified initial reactions in root cells after AHL exposures using physiological, staining, and electrophysiological methods. Treatment with short- and long-chain AHLs modulated plant growth and branched root architecture and induced nitric oxide (NO) accumulation in the calyptra and root elongation zone of excised roots in an AHL derivative-independent way. Additionally, C6- and C8-HSL treatments stimulated K+ uptake in root cells only at certain concentrations, whereas all tested concentrations of C12-HSL induced K+ uptake. In further experiments, C8-HSL promoted membrane hyperpolarization in epidermal root cells. Thus, we conclude AHLs promote plant growth and lateral root formation, and cause NO accumulation as an early response to AHLs. Furthermore, the AHL-mediated membrane hyperpolarization is leading to increased K+ uptake of the root tissue.
AB - While colonizing the rhizosphere, bacterial intra- and inter-specific communication is accomplished by N-Acyl-homoserine-lactones (AHLs) in a density-dependent manner. Moreover, plants are naturally exposed to AHLs and respond with tissue-specificity. In the present study, we investigated the influence of N-hexanoyl- (C6-HSL), N-octanoyl- (C8-HSL) and N-dodecanoyl-D/L-homoserine lactone (C12-HSL) on growth and root development in barley (Hordeum vulgare L.), and identified initial reactions in root cells after AHL exposures using physiological, staining, and electrophysiological methods. Treatment with short- and long-chain AHLs modulated plant growth and branched root architecture and induced nitric oxide (NO) accumulation in the calyptra and root elongation zone of excised roots in an AHL derivative-independent way. Additionally, C6- and C8-HSL treatments stimulated K+ uptake in root cells only at certain concentrations, whereas all tested concentrations of C12-HSL induced K+ uptake. In further experiments, C8-HSL promoted membrane hyperpolarization in epidermal root cells. Thus, we conclude AHLs promote plant growth and lateral root formation, and cause NO accumulation as an early response to AHLs. Furthermore, the AHL-mediated membrane hyperpolarization is leading to increased K+ uptake of the root tissue.
KW - Hordeum vulgare
KW - Membrane hyperpolarization
KW - Nitric oxide
KW - Nutrient uptake
KW - Quorum sensing
KW - Root development
UR - http://www.scopus.com/inward/record.url?scp=84991773726&partnerID=8YFLogxK
U2 - 10.1016/j.plantsci.2016.09.014
DO - 10.1016/j.plantsci.2016.09.014
M3 - Article
C2 - 27968982
AN - SCOPUS:84991773726
SN - 0168-9452
VL - 253
SP - 130
EP - 140
JO - Plant Science
JF - Plant Science
ER -