mGluR activation reveals a tonic NMDA component in inflammatory hyperalgesia

Sarah J. Boxall; Achim Berthele; Thomas R. Tölle; Walter Zieglgänsberger; Laszlo Urban

doi:10.1097/00001756-199804200-00044

mGluR activation reveals a tonic NMDA component in inflammatory hyperalgesia

Sarah J. Boxall, Achim Berthele, Thomas R. Tölle, Walter Zieglgänsberger, Laszlo Urban

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

METABOTROPIC glutamate receptors (mGluRs) have been shown to contribute to nociceptive processing in the spinal cord. We have investigated the pharmacology of the mGluR agonist (1S,3R)-ACPD during inflammatory hyperalgesia in an in vitro preparation of the juvenile rat hemisected spinal cord. Superfusion of (1S,3R)-ACPD produced a concentration-dependent ventral root depolarization in naive and hyperalgesic animals with no significant difference in EC₅₀ values (55.5 ± 6.36 μM and 51.0 ± 5.76 μM, respectively, n = 4). However, the amplitude of the maximum response was significantly enhanced by 23% in hyperalgesic compared with naive animals. The NMDA receptor antagonist D-AP5 reversed this effect, leaving the (1S,3R)- ACPD dose-response curve unchanged in naive animals. These results suggest a tonic NMDA component in the spinal cord during inflammatory hyperalgesia.

Original language	English
Pages (from-to)	1201-1203
Number of pages	3
Journal	NeuroReport
Volume	9
Issue number	6
DOIs	https://doi.org/10.1097/00001756-199804200-00044
State	Published - 20 Apr 1998

Keywords

(1S,3R)-ACPD
Inflammation
Metabotropic glutamate receptors
Spinal cord

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10.1097/00001756-199804200-00044

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title = "mGluR activation reveals a tonic NMDA component in inflammatory hyperalgesia",

abstract = "METABOTROPIC glutamate receptors (mGluRs) have been shown to contribute to nociceptive processing in the spinal cord. We have investigated the pharmacology of the mGluR agonist (1S,3R)-ACPD during inflammatory hyperalgesia in an in vitro preparation of the juvenile rat hemisected spinal cord. Superfusion of (1S,3R)-ACPD produced a concentration-dependent ventral root depolarization in naive and hyperalgesic animals with no significant difference in EC50 values (55.5 ± 6.36 μM and 51.0 ± 5.76 μM, respectively, n = 4). However, the amplitude of the maximum response was significantly enhanced by 23% in hyperalgesic compared with naive animals. The NMDA receptor antagonist D-AP5 reversed this effect, leaving the (1S,3R)- ACPD dose-response curve unchanged in naive animals. These results suggest a tonic NMDA component in the spinal cord during inflammatory hyperalgesia.",

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author = "Boxall, {Sarah J.} and Achim Berthele and T{\"o}lle, {Thomas R.} and Walter Zieglg{\"a}nsberger and Laszlo Urban",

year = "1998",

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T1 - mGluR activation reveals a tonic NMDA component in inflammatory hyperalgesia

AU - Boxall, Sarah J.

AU - Berthele, Achim

AU - Tölle, Thomas R.

AU - Zieglgänsberger, Walter

AU - Urban, Laszlo

PY - 1998/4/20

Y1 - 1998/4/20

N2 - METABOTROPIC glutamate receptors (mGluRs) have been shown to contribute to nociceptive processing in the spinal cord. We have investigated the pharmacology of the mGluR agonist (1S,3R)-ACPD during inflammatory hyperalgesia in an in vitro preparation of the juvenile rat hemisected spinal cord. Superfusion of (1S,3R)-ACPD produced a concentration-dependent ventral root depolarization in naive and hyperalgesic animals with no significant difference in EC50 values (55.5 ± 6.36 μM and 51.0 ± 5.76 μM, respectively, n = 4). However, the amplitude of the maximum response was significantly enhanced by 23% in hyperalgesic compared with naive animals. The NMDA receptor antagonist D-AP5 reversed this effect, leaving the (1S,3R)- ACPD dose-response curve unchanged in naive animals. These results suggest a tonic NMDA component in the spinal cord during inflammatory hyperalgesia.

AB - METABOTROPIC glutamate receptors (mGluRs) have been shown to contribute to nociceptive processing in the spinal cord. We have investigated the pharmacology of the mGluR agonist (1S,3R)-ACPD during inflammatory hyperalgesia in an in vitro preparation of the juvenile rat hemisected spinal cord. Superfusion of (1S,3R)-ACPD produced a concentration-dependent ventral root depolarization in naive and hyperalgesic animals with no significant difference in EC50 values (55.5 ± 6.36 μM and 51.0 ± 5.76 μM, respectively, n = 4). However, the amplitude of the maximum response was significantly enhanced by 23% in hyperalgesic compared with naive animals. The NMDA receptor antagonist D-AP5 reversed this effect, leaving the (1S,3R)- ACPD dose-response curve unchanged in naive animals. These results suggest a tonic NMDA component in the spinal cord during inflammatory hyperalgesia.

KW - (1S,3R)-ACPD

KW - Inflammation

KW - Metabotropic glutamate receptors

KW - Spinal cord

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mGluR activation reveals a tonic NMDA component in inflammatory hyperalgesia

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Keywords

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