TY - JOUR
T1 - Methyl jasmonate treatment induces changes in fruit ripening by modifying the expression of several ripening genes in Fragaria chiloensis fruit
AU - Concha, Cristóbal M.
AU - Figueroa, Nicolás E.
AU - Poblete, Leticia A.
AU - Oñate, Felipe A.
AU - Schwab, Wilfried
AU - Figueroa, Carlos R.
N1 - Funding Information:
C.R.F. thankfully acknowledges the “Deutscher Akademischer Austausch Dienst” for a research fellowship (DAAD A/11/00747) and the Group of Biotechnology of Natural Products at the Technical University of Munich for a research stay. Additionally, C.R.F. thanks Dr. M. A. Moya-León (University of Talca, Chile) for providing the F. chiloensis genomic libraries. This work was supported by the FONDECYT 11110171 and 3100031 projects.
PY - 2013/9
Y1 - 2013/9
N2 - To investigate the role of jasmonates (JAs) in the ripening of Fragaria chiloensis fruit, two concentrations of methyl jasmonate (MeJA, 10 and 100μM) were evaluated at 2, 5 and 9dusing an invitro ripening system. Fruit quality parameters; the contents of anthocyanin, lignin and cell wall polymers; and the transcriptional profiles of several ripening-related genes were analyzed. MeJA accelerated fruit ripening by means of a transitory increase in the soluble solid content/titratable acidity ratio, anthocyanin accumulation and an increase in softening at day 5. The expression of several phenylpropanoid-related genes, primarily those associated with anthocyanin biosynthesis, was increased under MeJA treatment, which correlated with an increased accumulation of anthocyanin. MeJA also altered the expression profiles of some cell wall-modifying genes, namely, EG1 and XTH1, and these changes correlated with a transient reduction in the firmness of MeJA-treated fruits. MeJA-responsive elements were observed in the promoter region of the EG1 gene. MeJA also increased the expression of LOX, AOS and OPR3, genes involved in the biosynthesis of JAs, and these changes correlated with the transient activation of fruit ripening observed. Conversely, the expression of ethylene and lignin biosynthesis genes (ACS, ACO, CAD and POD27) increased in MeJA-treated fruits at day 9. The present findings suggest that JAs promote the ripening of non-climacteric fruits through their involvement in anthocyanin accumulation, cell wall modification and the biosynthesis of ethylene and JAs.
AB - To investigate the role of jasmonates (JAs) in the ripening of Fragaria chiloensis fruit, two concentrations of methyl jasmonate (MeJA, 10 and 100μM) were evaluated at 2, 5 and 9dusing an invitro ripening system. Fruit quality parameters; the contents of anthocyanin, lignin and cell wall polymers; and the transcriptional profiles of several ripening-related genes were analyzed. MeJA accelerated fruit ripening by means of a transitory increase in the soluble solid content/titratable acidity ratio, anthocyanin accumulation and an increase in softening at day 5. The expression of several phenylpropanoid-related genes, primarily those associated with anthocyanin biosynthesis, was increased under MeJA treatment, which correlated with an increased accumulation of anthocyanin. MeJA also altered the expression profiles of some cell wall-modifying genes, namely, EG1 and XTH1, and these changes correlated with a transient reduction in the firmness of MeJA-treated fruits. MeJA-responsive elements were observed in the promoter region of the EG1 gene. MeJA also increased the expression of LOX, AOS and OPR3, genes involved in the biosynthesis of JAs, and these changes correlated with the transient activation of fruit ripening observed. Conversely, the expression of ethylene and lignin biosynthesis genes (ACS, ACO, CAD and POD27) increased in MeJA-treated fruits at day 9. The present findings suggest that JAs promote the ripening of non-climacteric fruits through their involvement in anthocyanin accumulation, cell wall modification and the biosynthesis of ethylene and JAs.
KW - Anthocyanin and lignin biosynthesis
KW - Cell wall modification
KW - Chilean strawberry
KW - Fruit ripening
KW - Gene expression analysis
KW - Jasmonates
UR - http://www.scopus.com/inward/record.url?scp=84880307361&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2013.06.008
DO - 10.1016/j.plaphy.2013.06.008
M3 - Article
C2 - 23835361
AN - SCOPUS:84880307361
SN - 0981-9428
VL - 70
SP - 433
EP - 444
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
ER -