Metatranscriptomic analysis of modified atmosphere packaged poultry meat enables prediction of Brochothrix thermosphacta and Carnobacterium divergens in situ metabolism

Linda Höll, Maik Hilgarth, Andreas J. Geissler, Jürgen Behr, Rudi F. Vogel

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

In this study, in situ-expressed metabolic routes of Brochothrix (B.) thermosphacta and Carnobacterium (C.) divergens were evaluated based on a metatranscriptomic dataset from bacteria growing on MAP chicken meat (O2/CO2; N2/CO2). Both species exhibited no (C. divergens) or minor transcription regulation (B. thermosphacta) within their main metabolic routes in response to different atmospheres. Both employ pathways related to glucose and ribose. Gluconeogenesis from lipid-borne glycerol is active in the progressing lack of carbohydrates. Pyruvate fates in both species comprise lactate, ethanol, acetate, CO2, formate, C4-compounds and H2O2 (only B. thermosphacta). Both species express genes for a minimal aerobic respiratory chain, but do not possess the genetic setting for a functional citric acid cycle. While products of carbohydrate and glycerol metabolism display mild to medium sensorial off-characteristics, predicted end products of their amino acid metabolism comprise, e.g., isobutyrate and isovalerate (B. thermosphacta) or cadaverine and tyramine (C. divergens) as potent spoilage compounds.

Original languageEnglish
Pages (from-to)1945-1955
Number of pages11
JournalArchives of Microbiology
Volume202
Issue number7
DOIs
StatePublished - 1 Sep 2020

Keywords

  • Brochothrix thermosphacta
  • Carnobacterium divergens
  • Meat spoilage
  • Metatranscriptomics
  • Modified atmosphere packaging

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