Metabolite monitoring in plants with double-quantum filtered chemical shift imaging

Kristina Wolf; Annette Van Der Toorn; Klaus Hartmann; Lukas Schreiber; Wilfried Schwab; Axel Haase; Gerhard Bringmann

doi:10.1093/jexbot/51.353.2109

Metabolite monitoring in plants with double-quantum filtered chemical shift imaging

Kristina Wolf, Annette Van Der Toorn, Klaus Hartmann, Lukas Schreiber, Wilfried Schwab, Axel Haase, Gerhard Bringmann

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

¹H spectroscopic imaging in combination with double-quantum filtering with magnetic field gradients is used for in vivo applications in humans and animals. Because of its high selectivity and strong reduction of water signal it is also a useful tool for monitoring the distribution of specific metabolites in plants. The development and application of a double-quantum selective spectroscopic imaging sequence for detecting the sucrose distribution in the stem of sugar cane (Saccharum officinarum L.) is described. The results show that local differences in sucrose distribution can be detected non-invasively with a resolution of 0.4 × 0.4 × 6 mm₃ to 0.645 × 0.645 × 6 mm³.

Original language	English
Pages (from-to)	2109-2117
Number of pages	9
Journal	Journal of Experimental Botany
Volume	51
Issue number	353
DOIs	https://doi.org/10.1093/jexbot/51.353.2109
State	Published - 2000
Externally published	Yes

Keywords

Localized NMR spectroscopy
Metabolite monitoring
NMR imaging
Sugar cane

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10.1093/jexbot/51.353.2109

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@article{f4afb16e6fdf42c0b77ae365af3e24d6,

title = "Metabolite monitoring in plants with double-quantum filtered chemical shift imaging",

abstract = "1H spectroscopic imaging in combination with double-quantum filtering with magnetic field gradients is used for in vivo applications in humans and animals. Because of its high selectivity and strong reduction of water signal it is also a useful tool for monitoring the distribution of specific metabolites in plants. The development and application of a double-quantum selective spectroscopic imaging sequence for detecting the sucrose distribution in the stem of sugar cane (Saccharum officinarum L.) is described. The results show that local differences in sucrose distribution can be detected non-invasively with a resolution of 0.4 × 0.4 × 6 mm3 to 0.645 × 0.645 × 6 mm3.",

keywords = "Localized NMR spectroscopy, Metabolite monitoring, NMR imaging, Sugar cane",

author = "Kristina Wolf and {Van Der Toorn}, Annette and Klaus Hartmann and Lukas Schreiber and Wilfried Schwab and Axel Haase and Gerhard Bringmann",

note = "Funding Information: This work was funded by the Deutsche Forschungsgemeinschaft (`Graduiertenkolleg NMR in vivo und in vitro f{\"u}r die biologische und medizinische Grundlagenforschung') and the Fonds der Chemischen Industrie. KW thanks the Studienstiftung des Deutschen Volkes and AvdT the European Union for generous fellowships. The authors wish to thank Markus Rokitta, Michael Szimtenings, Wilfried Landsch{\"u}tz, and Titus Lanz for designing and building the probeheads and Dr Markus R{\"u}ckert for fruitful discussions.",

year = "2000",

doi = "10.1093/jexbot/51.353.2109",

language = "English",

volume = "51",

pages = "2109--2117",

journal = "Journal of Experimental Botany",

issn = "0022-0957",

publisher = "Oxford University Press",

number = "353",

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TY - JOUR

T1 - Metabolite monitoring in plants with double-quantum filtered chemical shift imaging

AU - Wolf, Kristina

AU - Van Der Toorn, Annette

AU - Hartmann, Klaus

AU - Schreiber, Lukas

AU - Schwab, Wilfried

AU - Haase, Axel

AU - Bringmann, Gerhard

N1 - Funding Information: This work was funded by the Deutsche Forschungsgemeinschaft (`Graduiertenkolleg NMR in vivo und in vitro für die biologische und medizinische Grundlagenforschung') and the Fonds der Chemischen Industrie. KW thanks the Studienstiftung des Deutschen Volkes and AvdT the European Union for generous fellowships. The authors wish to thank Markus Rokitta, Michael Szimtenings, Wilfried Landschütz, and Titus Lanz for designing and building the probeheads and Dr Markus Rückert for fruitful discussions.

PY - 2000

Y1 - 2000

N2 - 1H spectroscopic imaging in combination with double-quantum filtering with magnetic field gradients is used for in vivo applications in humans and animals. Because of its high selectivity and strong reduction of water signal it is also a useful tool for monitoring the distribution of specific metabolites in plants. The development and application of a double-quantum selective spectroscopic imaging sequence for detecting the sucrose distribution in the stem of sugar cane (Saccharum officinarum L.) is described. The results show that local differences in sucrose distribution can be detected non-invasively with a resolution of 0.4 × 0.4 × 6 mm3 to 0.645 × 0.645 × 6 mm3.

AB - 1H spectroscopic imaging in combination with double-quantum filtering with magnetic field gradients is used for in vivo applications in humans and animals. Because of its high selectivity and strong reduction of water signal it is also a useful tool for monitoring the distribution of specific metabolites in plants. The development and application of a double-quantum selective spectroscopic imaging sequence for detecting the sucrose distribution in the stem of sugar cane (Saccharum officinarum L.) is described. The results show that local differences in sucrose distribution can be detected non-invasively with a resolution of 0.4 × 0.4 × 6 mm3 to 0.645 × 0.645 × 6 mm3.

KW - Localized NMR spectroscopy

KW - Metabolite monitoring

KW - NMR imaging

KW - Sugar cane

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DO - 10.1093/jexbot/51.353.2109

M3 - Article

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AN - SCOPUS:0034493517

SN - 0022-0957

VL - 51

SP - 2109

EP - 2117

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - 353

ER -

Metabolite monitoring in plants with double-quantum filtered chemical shift imaging

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Keywords

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