Metabolism of phenanthrene, benz[a]anthracene, benzo[a]pyrene, chrysene and benzo[c]phenanthrene by eight cDNA-expressed human and rat cytochromes P450

Jürgen Jacob, Johannes Doehmer, Gernot Grimmer, Volker Soballa, Gottfried Raab, Albrecht Seidel, Helmut Greim

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Phenanthrene, benz[a]anthracene, chrysene, benzo[c]phenanthrene, and benzo[a]pyrene have been studied for their regiospecific oxidation by five human (1A1, 1A2, 2A6, 2E1, 3A4) and three rat (1A1, 1A2, 2B1) CYP isoforms. All substrates are preferentially metabolized by CYP1A1 and CYP1A2 in human and rat. Other isoforms play a minor role if at all. Significant differences between human and rat CYP isoforms can be recognized with regard to the regiospecific oxidation of PAH. For instance, K-region oxidation is more pronounced in rat than in human CYP1A1 and CYP1A2. Hence, extrapolation from metabolism studies in rodents to human may be limited.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalPolycyclic Aromatic Compounds
Volume10
Issue number1-4
DOIs
StatePublished - 1996
Externally publishedYes

Keywords

  • Benz[a]anthracene
  • Benzo[a]pyrene
  • Benzo[c]phenanthrene
  • Chrysene
  • Cyp450 isoforms
  • Phenanthrene

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