Metabolic and process engineering on the edge—Rhamnolipids are a true challenge: A review

Melanie Filbig, Sonja Kubicki, Isabel Bator, Rudolf Hausmann, Lars Mathias Blank, Marius Henkel, Stephan Thies, Till Tiso

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations


Recombinant rhamnolipid production circumvents challenges connected to the usage of pathogenic wild-type producers such as Pseudomonas aeruginosa. In order to construct an efficient recombinant strain, two major elements are needed: an optimized expression cassette for the heterologous genes and a chassis cell, optimized for rhamnolipid production. Methods and approaches for the design of expression cassettes and chassis are presented here. With a performing whole-cell catalyst in hand, efficient rhamnolipid production requires advanced process engineering. Especially with heavily foaming substances as rhamnolipids, much attention has to be turned on fermentation and downstream processing. For foam control different technologies such as mechanical and chemical foam collapsing, foam fractionation, and bubble free aeration are described. The recent approaches summarized here are evidence of a vivid research community investigating the many different facets of recombinant rhamnolipid production using P. putida.

Original languageEnglish
Title of host publicationBiosurfactants
Subtitle of host publicationResearch and Development
Number of pages25
ISBN (Electronic)9780323916974
StatePublished - 1 Jan 2023
Externally publishedYes


  • Downstream processing
  • Expression cassette
  • Fermentation
  • Foam
  • Genetic engineering
  • Metabolic engineering
  • Process development
  • Promoters
  • Pseudomonas putida
  • Rhamnolipids


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