Metabolic activation of haloalkanes and tests in vitro for mutagenicity

H. Uehleke; Therese Werner; H. Greim; Maria Kramer

doi:10.3109/00498257709035798

Metabolic activation of haloalkanes and tests in vitro for mutagenicity

H. Uehleke
, Therese Werner
, H. Greim
, Maria Kramer

Institute of Pharmacology
German Health Office
University of Tübingen
Helmholtz Zentrum München German Research Center for Environmental Health

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

1. During incubation of ¹⁴CCl₄, ¹⁴CHCl₃, [¹⁴C]halothane, or ¹⁴CCl₃F with liver microsomes and NADPH, considerable radioactivity is bound irreversibly to endoplasmic protein and lipid. However, no ¹⁴C was detected in the ribo-somal RNA. 2. None of the four haloalkanes studied induced mutations after incubation with liver microsomes and the bacterial tester strains S. typhimurium TA 1535 and TA 1538. 3. Very low or no activity was associated with soluble protein or RNA added to incubation mixtures of the four haloalkanes with liver microsomes.

Original language	English
Pages (from-to)	393-400
Number of pages	8
Journal	Xenobiotica
Volume	7
Issue number	7
DOIs	https://doi.org/10.3109/00498257709035798
State	Published - 1977
Externally published	Yes

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title = "Metabolic activation of haloalkanes and tests in vitro for mutagenicity",

abstract = "1. During incubation of 14CCl4, 14CHCl3, [14C]halothane, or 14CCl3F with liver microsomes and NADPH, considerable radioactivity is bound irreversibly to endoplasmic protein and lipid. However, no 14C was detected in the ribo-somal RNA. 2. None of the four haloalkanes studied induced mutations after incubation with liver microsomes and the bacterial tester strains S. typhimurium TA 1535 and TA 1538. 3. Very low or no activity was associated with soluble protein or RNA added to incubation mixtures of the four haloalkanes with liver microsomes.",

author = "H. Uehleke and Therese Werner and H. Greim and Maria Kramer",

year = "1977",

doi = "10.3109/00498257709035798",

language = "English",

volume = "7",

pages = "393--400",

journal = "Xenobiotica",

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publisher = "Taylor and Francis Ltd.",

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T1 - Metabolic activation of haloalkanes and tests in vitro for mutagenicity

AU - Uehleke, H.

AU - Werner, Therese

AU - Greim, H.

AU - Kramer, Maria

PY - 1977

Y1 - 1977

N2 - 1. During incubation of 14CCl4, 14CHCl3, [14C]halothane, or 14CCl3F with liver microsomes and NADPH, considerable radioactivity is bound irreversibly to endoplasmic protein and lipid. However, no 14C was detected in the ribo-somal RNA. 2. None of the four haloalkanes studied induced mutations after incubation with liver microsomes and the bacterial tester strains S. typhimurium TA 1535 and TA 1538. 3. Very low or no activity was associated with soluble protein or RNA added to incubation mixtures of the four haloalkanes with liver microsomes.

AB - 1. During incubation of 14CCl4, 14CHCl3, [14C]halothane, or 14CCl3F with liver microsomes and NADPH, considerable radioactivity is bound irreversibly to endoplasmic protein and lipid. However, no 14C was detected in the ribo-somal RNA. 2. None of the four haloalkanes studied induced mutations after incubation with liver microsomes and the bacterial tester strains S. typhimurium TA 1535 and TA 1538. 3. Very low or no activity was associated with soluble protein or RNA added to incubation mixtures of the four haloalkanes with liver microsomes.

UR - https://www.scopus.com/pages/publications/0017397030

U2 - 10.3109/00498257709035798

DO - 10.3109/00498257709035798

M3 - Article

C2 - 329590

AN - SCOPUS:0017397030

SN - 0049-8254

VL - 7

SP - 393

EP - 400

JO - Xenobiotica

JF - Xenobiotica

IS - 7

ER -

Metabolic activation of haloalkanes and tests in vitro for mutagenicity

Abstract

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