Membrane Hsp70-supported cell-to-cell connections via tunneling nanotubes revealed by live-cell STED nanoscopy

Judith Reindl, Maxim Shevtsov, Günther Dollinger, Stefan Stangl, Gabriele Multhoff

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Heat shock protein Hsp70 (Hsp70) is found on the cell surface of a large variety of human and mouse tumor cell types including U87, GL261 glioblastoma, and 4T1 mammary carcinoma cells. We studied the role of membrane-bound Hsp70 (mHsp70) in the formation of cell-to-cell connections via tunneling nanotubes (TNTs) using live-cell STED nanoscopy. This technique allows the visualization of microstructures in the 100-nm range in the living cells. We could show that the presence of tumor-derived mHsp70 in TNTs with a diameter ranging from 120 to 140 nm predominantly originates from cholesterol-rich-microdomains containing the lipid compound globoyltriaosylceramide (Gb3). Under non-stress conditions, Hsp70 and Gb3 are structurally clustered in the membrane of TNTs of tumor cells that showed tumor type specific variations in the amount of cell-to-cell connection networks. Furthermore depletion of cholesterol and ionizing radiation as a stress factor results in a complete loss of Hsp70-containing TNTs.

Original languageEnglish
Pages (from-to)213-221
Number of pages9
JournalCell Stress and Chaperones
Volume24
Issue number1
DOIs
StatePublished - 1 Jan 2019
Externally publishedYes

Keywords

  • Cell-to-cell connection
  • Cellular networks
  • Live-cell STED
  • Membrane-bound Hsp70
  • Tunneling nanotubes

Fingerprint

Dive into the research topics of 'Membrane Hsp70-supported cell-to-cell connections via tunneling nanotubes revealed by live-cell STED nanoscopy'. Together they form a unique fingerprint.

Cite this