TY - JOUR
T1 - Medium Optimization by Genetic Algorithm for Continuous Production of Formate Dehydrogenase
AU - Weuster-Botz, D.
AU - Wandrey, C.
N1 - Funding Information:
The authors gratefully acknowledge excellent support in the laboratory from Mrs Weber, Research Centre Jiilich, Germany and the absolutely reliable operation of the pilot plant by Mr Paschold, Research Centre Jiilich, Germany. The authors thank the Central Analytical Laboratories (ZCH) of the Research Centre Jiilich, Germany for performing the nitrogen, phosphorus and sulphur analysis of the dry cell mass. Financial support for this work by Hoffmann-La Roche, Basel, Switzerland is gratefully acknowledged.
PY - 1995
Y1 - 1995
N2 - A continuous production process for formate dehydrogenase (FDH) production by the methylotrophic yeast Candida boidinii was improved by medium optimization in parallel shake-flask experiments. Using a genetic algorithm the concentrations of 14 medium components (trace elements and vitamins) were optimized within 125 experiments to give a maximum FDH activity with minimum use of nutrients salts (full experimental plan: 10114 experiments!). Employing this optimized medium composition in a 200-litre pilot-scale experiment for continuous methanol-controlled FDH production, a high specific FDH activity of 70 U/g dry cell mass was achieved at a residence time of 15 h. Compared to results with the medium used previously, under the same operating conditions, FDH space-time yield and FDH activity were thus improved by 50% from 100 to 150 U/litre/h and 1550 to 2300 U/litre, respectively.
AB - A continuous production process for formate dehydrogenase (FDH) production by the methylotrophic yeast Candida boidinii was improved by medium optimization in parallel shake-flask experiments. Using a genetic algorithm the concentrations of 14 medium components (trace elements and vitamins) were optimized within 125 experiments to give a maximum FDH activity with minimum use of nutrients salts (full experimental plan: 10114 experiments!). Employing this optimized medium composition in a 200-litre pilot-scale experiment for continuous methanol-controlled FDH production, a high specific FDH activity of 70 U/g dry cell mass was achieved at a residence time of 15 h. Compared to results with the medium used previously, under the same operating conditions, FDH space-time yield and FDH activity were thus improved by 50% from 100 to 150 U/litre/h and 1550 to 2300 U/litre, respectively.
UR - https://www.scopus.com/pages/publications/0028871023
U2 - 10.1016/0032-9592(94)00036-0
DO - 10.1016/0032-9592(94)00036-0
M3 - Article
AN - SCOPUS:0028871023
SN - 1359-5113
VL - 30
SP - 563
EP - 571
JO - Process Biochemistry
JF - Process Biochemistry
IS - 6
ER -