Mediating specific cell adhesion to low-adhesive diblock copolymers by instant modification with cyclic RGD peptides

E. Lieb; M. Hacker; J. Tessmar; L. A. Kunz-Schughart; J. Fiedler; C. Dahmen; U. Hersel; H. Kessler; M. B. Schulz; A. Göpferich

doi:10.1016/j.biomaterials.2004.07.010

Mediating specific cell adhesion to low-adhesive diblock copolymers by instant modification with cyclic RGD peptides

E. Lieb, M. Hacker, J. Tessmar, L. A. Kunz-Schughart, J. Fiedler, C. Dahmen, U. Hersel, H. Kessler, M. B. Schulz, A. Göpferich

TUM Emeriti of Excellence

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

One promising strategy to control the interactions between biomaterial surfaces and attaching cells involves the covalent grafting of adhesion peptides to polymers on which protein adsorption, which mediates unspecific cell adhesion, is essentially suppressed. This study demonstrates a surface modification concept for the covalent anchoring of RGD peptides to reactive diblock copolymers based on monoamine poly(ethylene glycol)-block-poly(D,L- lactic acid) (H ₂N-PEG-PLA). Films of both the amine-reactive (ST-NH-PEG ₂PLA ₂₀) and the thiol-reactive derivative (MP-NH-PEG ₂PLA ₄₀) were modified with cyclic αvβ3/αvβ5 integrin subtype specific RGD peptides simply by incubation of the films with buffered solutions of the peptides. Human osteoblasts known to express these integrins were used to determine cell-polymer interactions. The adhesion experiments revealed significantly increased cell numbers and cell spreading on the RGD-modified surfaces mediated by RGD-integrin-interactions.

Original language	English
Pages (from-to)	2333-2341
Number of pages	9
Journal	Biomaterials
Volume	26
Issue number	15
DOIs	https://doi.org/10.1016/j.biomaterials.2004.07.010
State	Published - May 2005

Keywords

Biomimetic material
Cell adhesion
Diblock copolymer
Integrin
Osteoblast
Poly(ethylene oxide)
Poly(lactic acid)
RGD peptide
Surface modification

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10.1016/j.biomaterials.2004.07.010

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title = "Mediating specific cell adhesion to low-adhesive diblock copolymers by instant modification with cyclic RGD peptides",

abstract = "One promising strategy to control the interactions between biomaterial surfaces and attaching cells involves the covalent grafting of adhesion peptides to polymers on which protein adsorption, which mediates unspecific cell adhesion, is essentially suppressed. This study demonstrates a surface modification concept for the covalent anchoring of RGD peptides to reactive diblock copolymers based on monoamine poly(ethylene glycol)-block-poly(D,L- lactic acid) (H 2N-PEG-PLA). Films of both the amine-reactive (ST-NH-PEG 2PLA 20) and the thiol-reactive derivative (MP-NH-PEG 2PLA 40) were modified with cyclic αvβ3/αvβ5 integrin subtype specific RGD peptides simply by incubation of the films with buffered solutions of the peptides. Human osteoblasts known to express these integrins were used to determine cell-polymer interactions. The adhesion experiments revealed significantly increased cell numbers and cell spreading on the RGD-modified surfaces mediated by RGD-integrin-interactions.",

keywords = "Biomimetic material, Cell adhesion, Diblock copolymer, Integrin, Osteoblast, Poly(ethylene oxide), Poly(lactic acid), RGD peptide, Surface modification",

author = "E. Lieb and M. Hacker and J. Tessmar and Kunz-Schughart, {L. A.} and J. Fiedler and C. Dahmen and U. Hersel and H. Kessler and Schulz, {M. B.} and A. G{\"o}pferich",

note = "Funding Information: The authors thank the Bayerische Forschungsstiftung for their financial support (ForTePro). Special thanks are due to Allison Dennis, Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA, for proof-reading.",

year = "2005",

month = may,

doi = "10.1016/j.biomaterials.2004.07.010",

language = "English",

volume = "26",

pages = "2333--2341",

journal = "Biomaterials",

issn = "0142-9612",

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T1 - Mediating specific cell adhesion to low-adhesive diblock copolymers by instant modification with cyclic RGD peptides

AU - Lieb, E.

AU - Hacker, M.

AU - Tessmar, J.

AU - Kunz-Schughart, L. A.

AU - Fiedler, J.

AU - Dahmen, C.

AU - Hersel, U.

AU - Kessler, H.

AU - Schulz, M. B.

AU - Göpferich, A.

N1 - Funding Information: The authors thank the Bayerische Forschungsstiftung for their financial support (ForTePro). Special thanks are due to Allison Dennis, Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA, for proof-reading.

PY - 2005/5

Y1 - 2005/5

N2 - One promising strategy to control the interactions between biomaterial surfaces and attaching cells involves the covalent grafting of adhesion peptides to polymers on which protein adsorption, which mediates unspecific cell adhesion, is essentially suppressed. This study demonstrates a surface modification concept for the covalent anchoring of RGD peptides to reactive diblock copolymers based on monoamine poly(ethylene glycol)-block-poly(D,L- lactic acid) (H 2N-PEG-PLA). Films of both the amine-reactive (ST-NH-PEG 2PLA 20) and the thiol-reactive derivative (MP-NH-PEG 2PLA 40) were modified with cyclic αvβ3/αvβ5 integrin subtype specific RGD peptides simply by incubation of the films with buffered solutions of the peptides. Human osteoblasts known to express these integrins were used to determine cell-polymer interactions. The adhesion experiments revealed significantly increased cell numbers and cell spreading on the RGD-modified surfaces mediated by RGD-integrin-interactions.

AB - One promising strategy to control the interactions between biomaterial surfaces and attaching cells involves the covalent grafting of adhesion peptides to polymers on which protein adsorption, which mediates unspecific cell adhesion, is essentially suppressed. This study demonstrates a surface modification concept for the covalent anchoring of RGD peptides to reactive diblock copolymers based on monoamine poly(ethylene glycol)-block-poly(D,L- lactic acid) (H 2N-PEG-PLA). Films of both the amine-reactive (ST-NH-PEG 2PLA 20) and the thiol-reactive derivative (MP-NH-PEG 2PLA 40) were modified with cyclic αvβ3/αvβ5 integrin subtype specific RGD peptides simply by incubation of the films with buffered solutions of the peptides. Human osteoblasts known to express these integrins were used to determine cell-polymer interactions. The adhesion experiments revealed significantly increased cell numbers and cell spreading on the RGD-modified surfaces mediated by RGD-integrin-interactions.

KW - Biomimetic material

KW - Cell adhesion

KW - Diblock copolymer

KW - Integrin

KW - Osteoblast

KW - Poly(ethylene oxide)

KW - Poly(lactic acid)

KW - RGD peptide

KW - Surface modification

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DO - 10.1016/j.biomaterials.2004.07.010

M3 - Article

C2 - 15585236

AN - SCOPUS:20844450559

SN - 0142-9612

VL - 26

SP - 2333

EP - 2341

JO - Biomaterials

JF - Biomaterials

IS - 15

ER -

Mediating specific cell adhesion to low-adhesive diblock copolymers by instant modification with cyclic RGD peptides

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Keywords

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