Mechanism of the decay of thymine triplets in DNA single strands

Bert M. Pilles; Dominik B. Bucher; Lizhe Liu; Pascale Clivio; Peter Gilch; Wolfgang Zinth; Wolfgang J. Schreier

doi:10.1021/jz500364g

Mechanism of the decay of thymine triplets in DNA single strands

Bert M. Pilles, Dominik B. Bucher, Lizhe Liu, Pascale Clivio, Peter Gilch, Wolfgang Zinth, Wolfgang J. Schreier

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

The decay of triplet states and the formation of cyclobutane pyrimidine dimers (CPDs) after UV excitation of the all-thymine oligomer (dT)₁₈ and the locked dinucleotide T_LpT_L were studied by nanosecond IR spectroscopy. IR marker bands characteristic for the CPD lesion and the triplet state were observed from ∼1 ns (time resolution of the setup) onward. The amplitudes of the CPD marker bands remain constant throughout the time range covered (up to 10 μs). The triplet decays with a time constant of ∼10 ns presumably via a biradical intermediate (lifetime ∼60 ns). This biradical has often been invoked as an intermediate for CPD formation via the triplet channel. The present results lend strong support to the existence of this intermediate, yet there is no indication that its decay contributes significantly to CPD formation.

Original language	English
Pages (from-to)	1616-1622
Number of pages	7
Journal	Journal of Physical Chemistry Letters
Volume	5
Issue number	9
DOIs	https://doi.org/10.1021/jz500364g
State	Published - 1 May 2014
Externally published	Yes

Keywords

DNA photolesions
DNA photophysics
cyclobutane pyrimidine dimer
ns TRIR spectroscopy
thymine triplet state

Access to Document

10.1021/jz500364g

Cite this

@article{4596bc0601844d039fb7f7d999da1611,

title = "Mechanism of the decay of thymine triplets in DNA single strands",

abstract = "The decay of triplet states and the formation of cyclobutane pyrimidine dimers (CPDs) after UV excitation of the all-thymine oligomer (dT)18 and the locked dinucleotide TLpTL were studied by nanosecond IR spectroscopy. IR marker bands characteristic for the CPD lesion and the triplet state were observed from ∼1 ns (time resolution of the setup) onward. The amplitudes of the CPD marker bands remain constant throughout the time range covered (up to 10 μs). The triplet decays with a time constant of ∼10 ns presumably via a biradical intermediate (lifetime ∼60 ns). This biradical has often been invoked as an intermediate for CPD formation via the triplet channel. The present results lend strong support to the existence of this intermediate, yet there is no indication that its decay contributes significantly to CPD formation.",

keywords = "DNA photolesions, DNA photophysics, cyclobutane pyrimidine dimer, ns TRIR spectroscopy, thymine triplet state",

author = "Pilles, {Bert M.} and Bucher, {Dominik B.} and Lizhe Liu and Pascale Clivio and Peter Gilch and Wolfgang Zinth and Schreier, {Wolfgang J.}",

year = "2014",

month = may,

day = "1",

doi = "10.1021/jz500364g",

language = "English",

volume = "5",

pages = "1616--1622",

journal = "Journal of Physical Chemistry Letters",

issn = "1948-7185",

publisher = "American Chemical Society",

number = "9",

}

TY - JOUR

T1 - Mechanism of the decay of thymine triplets in DNA single strands

AU - Pilles, Bert M.

AU - Bucher, Dominik B.

AU - Liu, Lizhe

AU - Clivio, Pascale

AU - Gilch, Peter

AU - Zinth, Wolfgang

AU - Schreier, Wolfgang J.

PY - 2014/5/1

Y1 - 2014/5/1

N2 - The decay of triplet states and the formation of cyclobutane pyrimidine dimers (CPDs) after UV excitation of the all-thymine oligomer (dT)18 and the locked dinucleotide TLpTL were studied by nanosecond IR spectroscopy. IR marker bands characteristic for the CPD lesion and the triplet state were observed from ∼1 ns (time resolution of the setup) onward. The amplitudes of the CPD marker bands remain constant throughout the time range covered (up to 10 μs). The triplet decays with a time constant of ∼10 ns presumably via a biradical intermediate (lifetime ∼60 ns). This biradical has often been invoked as an intermediate for CPD formation via the triplet channel. The present results lend strong support to the existence of this intermediate, yet there is no indication that its decay contributes significantly to CPD formation.

AB - The decay of triplet states and the formation of cyclobutane pyrimidine dimers (CPDs) after UV excitation of the all-thymine oligomer (dT)18 and the locked dinucleotide TLpTL were studied by nanosecond IR spectroscopy. IR marker bands characteristic for the CPD lesion and the triplet state were observed from ∼1 ns (time resolution of the setup) onward. The amplitudes of the CPD marker bands remain constant throughout the time range covered (up to 10 μs). The triplet decays with a time constant of ∼10 ns presumably via a biradical intermediate (lifetime ∼60 ns). This biradical has often been invoked as an intermediate for CPD formation via the triplet channel. The present results lend strong support to the existence of this intermediate, yet there is no indication that its decay contributes significantly to CPD formation.

KW - DNA photolesions

KW - DNA photophysics

KW - cyclobutane pyrimidine dimer

KW - ns TRIR spectroscopy

KW - thymine triplet state

UR - http://www.scopus.com/inward/record.url?scp=84899817025&partnerID=8YFLogxK

U2 - 10.1021/jz500364g

DO - 10.1021/jz500364g

M3 - Article

AN - SCOPUS:84899817025

SN - 1948-7185

VL - 5

SP - 1616

EP - 1622

JO - Journal of Physical Chemistry Letters

JF - Journal of Physical Chemistry Letters

IS - 9

ER -

Mechanism of the decay of thymine triplets in DNA single strands

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this