MAX2-independent transcriptional responses to rac-GR24 in Lotus japonicus roots

Strigolactones (SLs) and smoke-derived Karrikins (KARs) are structurally similar butenolide compounds that control distinct aspects of plant development. They are perceived by two closely related α/β hydrolases D14 and KAI2, respectively. Responses to both molecules involve the F-box protein MAX2 that participates in the Skp1-Cullin-F-box (SCF) complex, which ubiquitylates developmental regulators of the SMXL family to mark them for degradation by the 26S proteasome, enabling SL and KAR responses. Current research on SL and KAR signaling uses the synthetic molecules rac-GR24, KAR₁ and KAR₂ for pharmacological treatments. In a previous microarray analysis, we observed transcriptional activation in response to rac-GR24 in Lotus japonicus seedling roots. We retested transcript accumulation of selected genes by quantitative PCR in the wild type and the max2-4 mutant, and found that surprisingly, a number of them respond to rac-GR24 in a MAX2-independent manner, and also respond in roots of d14 and kai2a kai2b double mutants. Thus, the synthetic compounds induce transcriptional responses independent of their perception by the canonical receptor complex.