Mass Spectrometry Imaging of atherosclerosis-affine Gadofluorine following Magnetic Resonance Imaging

Fabian Lohöfer; Rebecca Buchholz; Almut Glinzer; Katharina Huber; Helena Haas; Georgios Kaissis; Annette Feuchtinger; Michaela Aichler; Peter B. Sporns; Carsten Höltke; Miriam Stölting; Franz Schilling; René M. Botnar; Melanie A. Kimm; Cornelius Faber; Axel K. Walch; Alma Zernecke; Uwe Karst; Moritz Wildgruber

doi:10.1038/s41598-019-57075-6

Mass Spectrometry Imaging of atherosclerosis-affine Gadofluorine following Magnetic Resonance Imaging

Fabian Lohöfer, Rebecca Buchholz, Almut Glinzer, Katharina Huber, Helena Haas, Georgios Kaissis, Annette Feuchtinger, Michaela Aichler, Peter B. Sporns, Carsten Höltke, Miriam Stölting, Franz Schilling, René M. Botnar, Melanie A. Kimm, Cornelius Faber, Axel K. Walch, Alma Zernecke, Uwe Karst, Moritz Wildgruber

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Abstract

Molecular imaging of atherosclerosis by Magnetic Resonance Imaging (MRI) has been impaired by a lack of validation of the specific substrate responsible for the molecular imaging signal. We therefore aimed to investigate the additive value of mass spectrometry imaging (MSI) of atherosclerosis-affine Gadofluorine P for molecular MRI of atherosclerotic plaques. Atherosclerotic Ldlr^−/− mice were investigated by high-field MRI (7 T) at different time points following injection of atherosclerosis-affine Gadofluorine P as well as at different stages of atherosclerosis formation (4, 8, 16 and 20 weeks of HFD). At each imaging time point mice were immediately sacrificed after imaging and aortas were excised for mass spectrometry imaging: Matrix Assisted Laser Desorption Ionization (MALDI) Imaging and Laser Ablation – Inductively Coupled Plasma – Mass Spectrometry (LA-ICP-MS) imaging. Mass spectrometry imaging allowed to visualize the localization and measure the concentration of the MR imaging probe Gadofluorine P in plaque tissue ex vivo with high spatial resolution and thus adds novel and more target specific information to molecular MR imaging of atherosclerosis.

Original language	English
Article number	79
Journal	Scientific Reports
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41598-019-57075-6
State	Published - 1 Dec 2020
Externally published	Yes

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Lohöfer, F., Buchholz, R., Glinzer, A., Huber, K., Haas, H., Kaissis, G., Feuchtinger, A., Aichler, M., Sporns, P. B., Höltke, C., Stölting, M., Schilling, F., Botnar, R. M., Kimm, M. A., Faber, C., Walch, A. K., Zernecke, A., Karst, U., & Wildgruber, M. (2020). Mass Spectrometry Imaging of atherosclerosis-affine Gadofluorine following Magnetic Resonance Imaging. Scientific Reports, 10(1), Article 79. https://doi.org/10.1038/s41598-019-57075-6

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title = "Mass Spectrometry Imaging of atherosclerosis-affine Gadofluorine following Magnetic Resonance Imaging",

abstract = "Molecular imaging of atherosclerosis by Magnetic Resonance Imaging (MRI) has been impaired by a lack of validation of the specific substrate responsible for the molecular imaging signal. We therefore aimed to investigate the additive value of mass spectrometry imaging (MSI) of atherosclerosis-affine Gadofluorine P for molecular MRI of atherosclerotic plaques. Atherosclerotic Ldlr−/− mice were investigated by high-field MRI (7 T) at different time points following injection of atherosclerosis-affine Gadofluorine P as well as at different stages of atherosclerosis formation (4, 8, 16 and 20 weeks of HFD). At each imaging time point mice were immediately sacrificed after imaging and aortas were excised for mass spectrometry imaging: Matrix Assisted Laser Desorption Ionization (MALDI) Imaging and Laser Ablation – Inductively Coupled Plasma – Mass Spectrometry (LA-ICP-MS) imaging. Mass spectrometry imaging allowed to visualize the localization and measure the concentration of the MR imaging probe Gadofluorine P in plaque tissue ex vivo with high spatial resolution and thus adds novel and more target specific information to molecular MR imaging of atherosclerosis.",

author = "Fabian Loh{\"o}fer and Rebecca Buchholz and Almut Glinzer and Katharina Huber and Helena Haas and Georgios Kaissis and Annette Feuchtinger and Michaela Aichler and Sporns, {Peter B.} and Carsten H{\"o}ltke and Miriam St{\"o}lting and Franz Schilling and Botnar, {Ren{\'e} M.} and Kimm, {Melanie A.} and Cornelius Faber and Walch, {Axel K.} and Alma Zernecke and Uwe Karst and Moritz Wildgruber",

note = "Publisher Copyright: {\textcopyright} 2020, The Author(s).",

year = "2020",

month = dec,

day = "1",

doi = "10.1038/s41598-019-57075-6",

language = "English",

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Lohöfer, F, Buchholz, R, Glinzer, A, Huber, K, Haas, H, Kaissis, G, Feuchtinger, A, Aichler, M, Sporns, PB, Höltke, C, Stölting, M, Schilling, F, Botnar, RM, Kimm, MA, Faber, C, Walch, AK, Zernecke, A, Karst, U & Wildgruber, M 2020, 'Mass Spectrometry Imaging of atherosclerosis-affine Gadofluorine following Magnetic Resonance Imaging', Scientific Reports, vol. 10, no. 1, 79. https://doi.org/10.1038/s41598-019-57075-6

TY - JOUR

T1 - Mass Spectrometry Imaging of atherosclerosis-affine Gadofluorine following Magnetic Resonance Imaging

AU - Lohöfer, Fabian

AU - Buchholz, Rebecca

AU - Glinzer, Almut

AU - Huber, Katharina

AU - Haas, Helena

AU - Kaissis, Georgios

AU - Feuchtinger, Annette

AU - Aichler, Michaela

AU - Sporns, Peter B.

AU - Höltke, Carsten

AU - Stölting, Miriam

AU - Schilling, Franz

AU - Botnar, René M.

AU - Kimm, Melanie A.

AU - Faber, Cornelius

AU - Walch, Axel K.

AU - Zernecke, Alma

AU - Karst, Uwe

AU - Wildgruber, Moritz

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Molecular imaging of atherosclerosis by Magnetic Resonance Imaging (MRI) has been impaired by a lack of validation of the specific substrate responsible for the molecular imaging signal. We therefore aimed to investigate the additive value of mass spectrometry imaging (MSI) of atherosclerosis-affine Gadofluorine P for molecular MRI of atherosclerotic plaques. Atherosclerotic Ldlr−/− mice were investigated by high-field MRI (7 T) at different time points following injection of atherosclerosis-affine Gadofluorine P as well as at different stages of atherosclerosis formation (4, 8, 16 and 20 weeks of HFD). At each imaging time point mice were immediately sacrificed after imaging and aortas were excised for mass spectrometry imaging: Matrix Assisted Laser Desorption Ionization (MALDI) Imaging and Laser Ablation – Inductively Coupled Plasma – Mass Spectrometry (LA-ICP-MS) imaging. Mass spectrometry imaging allowed to visualize the localization and measure the concentration of the MR imaging probe Gadofluorine P in plaque tissue ex vivo with high spatial resolution and thus adds novel and more target specific information to molecular MR imaging of atherosclerosis.

AB - Molecular imaging of atherosclerosis by Magnetic Resonance Imaging (MRI) has been impaired by a lack of validation of the specific substrate responsible for the molecular imaging signal. We therefore aimed to investigate the additive value of mass spectrometry imaging (MSI) of atherosclerosis-affine Gadofluorine P for molecular MRI of atherosclerotic plaques. Atherosclerotic Ldlr−/− mice were investigated by high-field MRI (7 T) at different time points following injection of atherosclerosis-affine Gadofluorine P as well as at different stages of atherosclerosis formation (4, 8, 16 and 20 weeks of HFD). At each imaging time point mice were immediately sacrificed after imaging and aortas were excised for mass spectrometry imaging: Matrix Assisted Laser Desorption Ionization (MALDI) Imaging and Laser Ablation – Inductively Coupled Plasma – Mass Spectrometry (LA-ICP-MS) imaging. Mass spectrometry imaging allowed to visualize the localization and measure the concentration of the MR imaging probe Gadofluorine P in plaque tissue ex vivo with high spatial resolution and thus adds novel and more target specific information to molecular MR imaging of atherosclerosis.

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U2 - 10.1038/s41598-019-57075-6

DO - 10.1038/s41598-019-57075-6

M3 - Article

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AN - SCOPUS:85077712010

VL - 10

JO - Scientific Reports

JF - Scientific Reports

IS - 1

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ER -

Mass Spectrometry Imaging of atherosclerosis-affine Gadofluorine following Magnetic Resonance Imaging

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