Mapping of fire blight resistance in malus ×robusta 5 flowers following artificial inoculation

Andreas Peil; Christine Hübert; Annette Wensing; Mary Horner; Ofere Francis Emeriewen; Klaus Richter; Thomas Wöhner; David Chagné; Carolina Orellana-Torrejon; Munazza Saeed; Michela Troggio; Erika Stefani; Susan E. Gardiner; Magda Viola Hanke; Henryk Flachowsky; Vincent G.M. Bus

doi:10.1186/s12870-019-2154-7

Mapping of fire blight resistance in malus ×robusta 5 flowers following artificial inoculation

Andreas Peil, Christine Hübert, Annette Wensing, Mary Horner, Ofere Francis Emeriewen, Klaus Richter, Thomas Wöhner, David Chagné, Carolina Orellana-Torrejon, Munazza Saeed, Michela Troggio, Erika Stefani, Susan E. Gardiner, Magda Viola Hanke, Henryk Flachowsky, Vincent G.M. Bus

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Background: Although the most common path of infection for fire blight, a severe bacterial disease on apple, is via host plant flowers, quantitative trait loci (QTLs) for fire blight resistance to date have exclusively been mapped following shoot inoculation. It is not known whether the same mechanism underlies flower and shoot resistance. Results: We report the detection of a fire blight resistance QTL following independent artificial inoculation of flowers and shoots on two F1 segregating populations derived from crossing resistant Malus ×robusta 5 (Mr5) with susceptible 'Idared' and 'Royal Gala' in experimental orchards in Germany and New Zealand, respectively. QTL mapping of phenotypic datasets from artificial flower inoculation of the 'Idared' × Mr5 population with Erwinia amylovora over several years, and of the 'Royal Gala' × Mr5 population in a single year, revealed a single major QTL controlling floral fire blight resistance on linkage group 3 (LG3) of Mr5. This QTL corresponds to the QTL on LG3 reported previously for the 'Idared' × Mr5 and an 'M9' × Mr5 population following shoot inoculation in the glasshouse. Interval mapping of phenotypic data from shoot inoculations of subsets from both flower resistance populations re-confirmed that the resistance QTL is in the same position on LG3 of Mr5 as that for flower inoculation. These results provide strong evidence that fire blight resistance in Mr5 is controlled by a major QTL on LG3, independently of the mode of infection, rootstock and environment. Conclusions: This study demonstrates for the first time that resistance to fire blight caused by Erwinia amylovora is independent of the mode of inoculation at least in Malus ×robusta 5.

Original language	English
Article number	532
Journal	BMC Plant Biology
Volume	19
Issue number	1
DOIs	https://doi.org/10.1186/s12870-019-2154-7
State	Published - 2 Dec 2019
Externally published	Yes

Keywords

Erwinia amylovora
Fire blight resistance
Malus ×robusta 5
Orchard inoculation
QTL mapping

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10.1186/s12870-019-2154-7

Cite this

Peil, A., Hübert, C., Wensing, A., Horner, M., Emeriewen, O. F., Richter, K., Wöhner, T., Chagné, D., Orellana-Torrejon, C., Saeed, M., Troggio, M., Stefani, E., Gardiner, S. E., Hanke, M. V., Flachowsky, H., & Bus, V. G. M. (2019). Mapping of fire blight resistance in malus ×robusta 5 flowers following artificial inoculation. BMC Plant Biology, 19(1), Article 532. https://doi.org/10.1186/s12870-019-2154-7

@article{d0f55e1bd25c452db40897450c4d097d,

title = "Mapping of fire blight resistance in malus ×robusta 5 flowers following artificial inoculation",

abstract = "Background: Although the most common path of infection for fire blight, a severe bacterial disease on apple, is via host plant flowers, quantitative trait loci (QTLs) for fire blight resistance to date have exclusively been mapped following shoot inoculation. It is not known whether the same mechanism underlies flower and shoot resistance. Results: We report the detection of a fire blight resistance QTL following independent artificial inoculation of flowers and shoots on two F1 segregating populations derived from crossing resistant Malus ×robusta 5 (Mr5) with susceptible 'Idared' and 'Royal Gala' in experimental orchards in Germany and New Zealand, respectively. QTL mapping of phenotypic datasets from artificial flower inoculation of the 'Idared' × Mr5 population with Erwinia amylovora over several years, and of the 'Royal Gala' × Mr5 population in a single year, revealed a single major QTL controlling floral fire blight resistance on linkage group 3 (LG3) of Mr5. This QTL corresponds to the QTL on LG3 reported previously for the 'Idared' × Mr5 and an 'M9' × Mr5 population following shoot inoculation in the glasshouse. Interval mapping of phenotypic data from shoot inoculations of subsets from both flower resistance populations re-confirmed that the resistance QTL is in the same position on LG3 of Mr5 as that for flower inoculation. These results provide strong evidence that fire blight resistance in Mr5 is controlled by a major QTL on LG3, independently of the mode of infection, rootstock and environment. Conclusions: This study demonstrates for the first time that resistance to fire blight caused by Erwinia amylovora is independent of the mode of inoculation at least in Malus ×robusta 5.",

keywords = "Erwinia amylovora, Fire blight resistance, Malus ×robusta 5, Orchard inoculation, QTL mapping",

author = "Andreas Peil and Christine H{\"u}bert and Annette Wensing and Mary Horner and Emeriewen, {Ofere Francis} and Klaus Richter and Thomas W{\"o}hner and David Chagn{\'e} and Carolina Orellana-Torrejon and Munazza Saeed and Michela Troggio and Erika Stefani and Gardiner, {Susan E.} and Hanke, {Magda Viola} and Henryk Flachowsky and Bus, {Vincent G.M.}",

note = "Publisher Copyright: {\textcopyright} 2019 The Author(s).",

year = "2019",

month = dec,

day = "2",

doi = "10.1186/s12870-019-2154-7",

language = "English",

volume = "19",

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issn = "1471-2229",

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Peil, A, Hübert, C, Wensing, A, Horner, M, Emeriewen, OF, Richter, K, Wöhner, T, Chagné, D, Orellana-Torrejon, C, Saeed, M, Troggio, M, Stefani, E, Gardiner, SE, Hanke, MV, Flachowsky, H & Bus, VGM 2019, 'Mapping of fire blight resistance in malus ×robusta 5 flowers following artificial inoculation', BMC Plant Biology, vol. 19, no. 1, 532. https://doi.org/10.1186/s12870-019-2154-7

TY - JOUR

T1 - Mapping of fire blight resistance in malus ×robusta 5 flowers following artificial inoculation

AU - Peil, Andreas

AU - Hübert, Christine

AU - Wensing, Annette

AU - Horner, Mary

AU - Emeriewen, Ofere Francis

AU - Richter, Klaus

AU - Wöhner, Thomas

AU - Chagné, David

AU - Orellana-Torrejon, Carolina

AU - Saeed, Munazza

AU - Troggio, Michela

AU - Stefani, Erika

AU - Gardiner, Susan E.

AU - Hanke, Magda Viola

AU - Flachowsky, Henryk

AU - Bus, Vincent G.M.

PY - 2019/12/2

Y1 - 2019/12/2

N2 - Background: Although the most common path of infection for fire blight, a severe bacterial disease on apple, is via host plant flowers, quantitative trait loci (QTLs) for fire blight resistance to date have exclusively been mapped following shoot inoculation. It is not known whether the same mechanism underlies flower and shoot resistance. Results: We report the detection of a fire blight resistance QTL following independent artificial inoculation of flowers and shoots on two F1 segregating populations derived from crossing resistant Malus ×robusta 5 (Mr5) with susceptible 'Idared' and 'Royal Gala' in experimental orchards in Germany and New Zealand, respectively. QTL mapping of phenotypic datasets from artificial flower inoculation of the 'Idared' × Mr5 population with Erwinia amylovora over several years, and of the 'Royal Gala' × Mr5 population in a single year, revealed a single major QTL controlling floral fire blight resistance on linkage group 3 (LG3) of Mr5. This QTL corresponds to the QTL on LG3 reported previously for the 'Idared' × Mr5 and an 'M9' × Mr5 population following shoot inoculation in the glasshouse. Interval mapping of phenotypic data from shoot inoculations of subsets from both flower resistance populations re-confirmed that the resistance QTL is in the same position on LG3 of Mr5 as that for flower inoculation. These results provide strong evidence that fire blight resistance in Mr5 is controlled by a major QTL on LG3, independently of the mode of infection, rootstock and environment. Conclusions: This study demonstrates for the first time that resistance to fire blight caused by Erwinia amylovora is independent of the mode of inoculation at least in Malus ×robusta 5.

AB - Background: Although the most common path of infection for fire blight, a severe bacterial disease on apple, is via host plant flowers, quantitative trait loci (QTLs) for fire blight resistance to date have exclusively been mapped following shoot inoculation. It is not known whether the same mechanism underlies flower and shoot resistance. Results: We report the detection of a fire blight resistance QTL following independent artificial inoculation of flowers and shoots on two F1 segregating populations derived from crossing resistant Malus ×robusta 5 (Mr5) with susceptible 'Idared' and 'Royal Gala' in experimental orchards in Germany and New Zealand, respectively. QTL mapping of phenotypic datasets from artificial flower inoculation of the 'Idared' × Mr5 population with Erwinia amylovora over several years, and of the 'Royal Gala' × Mr5 population in a single year, revealed a single major QTL controlling floral fire blight resistance on linkage group 3 (LG3) of Mr5. This QTL corresponds to the QTL on LG3 reported previously for the 'Idared' × Mr5 and an 'M9' × Mr5 population following shoot inoculation in the glasshouse. Interval mapping of phenotypic data from shoot inoculations of subsets from both flower resistance populations re-confirmed that the resistance QTL is in the same position on LG3 of Mr5 as that for flower inoculation. These results provide strong evidence that fire blight resistance in Mr5 is controlled by a major QTL on LG3, independently of the mode of infection, rootstock and environment. Conclusions: This study demonstrates for the first time that resistance to fire blight caused by Erwinia amylovora is independent of the mode of inoculation at least in Malus ×robusta 5.

KW - Erwinia amylovora

KW - Fire blight resistance

KW - Malus ×robusta 5

KW - Orchard inoculation

KW - QTL mapping

UR - http://www.scopus.com/inward/record.url?scp=85075930393&partnerID=8YFLogxK

U2 - 10.1186/s12870-019-2154-7

DO - 10.1186/s12870-019-2154-7

M3 - Article

C2 - 31791233

AN - SCOPUS:85075930393

SN - 1471-2229

VL - 19

JO - BMC Plant Biology

JF - BMC Plant Biology

IS - 1

M1 - 532

ER -

Mapping of fire blight resistance in malus ×robusta 5 flowers following artificial inoculation

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Keywords

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