Mapping of a mouse mammary tumor virus integration site by retroviral LTR - Arbitrary polymerase chain reaction

Claus Casper; Christine Leib-Mösch; Brian Salmons; Walter H. Günzburg; Gaby Baumann; Heinz Höfler; Volker Erfle; Michael J. Atkinson

doi:10.1016/S0168-1702(98)00002-1

Mapping of a mouse mammary tumor virus integration site by retroviral LTR - Arbitrary polymerase chain reaction

Claus Casper, Christine Leib-Mösch, Brian Salmons, Walter H. Günzburg, Gaby Baumann, Heinz Höfler, Volker Erfle, Michael J. Atkinson

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The de novo integration of retroviral genomes within the mammalian genome is believed to contribute to the tumorigenic process. Integration may result in the disruption or inappropriate transcription of key regulatory genes. We describe the application of an arbitrarily primed PCR method for the mapping and cloning of genomic integration sites of the mouse mammary tumor virus (MMTV). We have amplified DNA sequences between a selected retroviral MMTV-LTR and random sites in the 3' flanking DNA. Using this technique we were able to visualize several proviral integration sites present in a MMTV-induced mammary tumor derived cell line that were absent from the germ line. Cloning and sequencing of the PCR product corresponding to one site established its identification as an unique 3' flanking sequence. Copyright (C) 1998 Elsevier Science B.V.

Original language	English
Pages (from-to)	207-215
Number of pages	9
Journal	Virus Research
Volume	54
Issue number	2
DOIs	https://doi.org/10.1016/S0168-1702(98)00002-1
State	Published - Apr 1998
Externally published	Yes

Keywords

Insertional mutagenesis
Mouse mammary tumor virus
Polymerase chain reaction
Retroviral LTR arbitrarily primed PCR (RELAP-PCR)
Retroviral integration site

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0168-1702(98)00002-1

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@article{2580bbd849b34303a2999b2ea942c7b9,

title = "Mapping of a mouse mammary tumor virus integration site by retroviral LTR - Arbitrary polymerase chain reaction",

abstract = "The de novo integration of retroviral genomes within the mammalian genome is believed to contribute to the tumorigenic process. Integration may result in the disruption or inappropriate transcription of key regulatory genes. We describe the application of an arbitrarily primed PCR method for the mapping and cloning of genomic integration sites of the mouse mammary tumor virus (MMTV). We have amplified DNA sequences between a selected retroviral MMTV-LTR and random sites in the 3' flanking DNA. Using this technique we were able to visualize several proviral integration sites present in a MMTV-induced mammary tumor derived cell line that were absent from the germ line. Cloning and sequencing of the PCR product corresponding to one site established its identification as an unique 3' flanking sequence. Copyright (C) 1998 Elsevier Science B.V.",

keywords = "Insertional mutagenesis, Mouse mammary tumor virus, Polymerase chain reaction, Retroviral LTR arbitrarily primed PCR (RELAP-PCR), Retroviral integration site",

author = "Claus Casper and Christine Leib-M{\"o}sch and Brian Salmons and G{\"u}nzburg, {Walter H.} and Gaby Baumann and Heinz H{\"o}fler and Volker Erfle and Atkinson, {Michael J.}",

note = "Funding Information: We are grateful to W. Gimbel, Institute of Molecular Virology for helpful discussions in establishing the RELAP-PCR technique, Rainer H. Dunkl for assistance with the cell culture. We also thank the AG BioDV of the GSF-research center, especially U. Linzner and T. Werner for providing oligonucleotide primers. T. Mietke, Institute of Microbiology, Technical University Munich, kindly provided a GR-mouse liver for DNA isolation. Supported by grants from the Commission of the European Commission CT95-0008b (M.J.A) and DFG (Deutsche Forschungs Gemeinschaft) Ca 165/1-1 (C.C.). ",

year = "1998",

month = apr,

doi = "10.1016/S0168-1702(98)00002-1",

language = "English",

volume = "54",

pages = "207--215",

journal = "Virus Research",

issn = "0168-1702",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Mapping of a mouse mammary tumor virus integration site by retroviral LTR - Arbitrary polymerase chain reaction

AU - Casper, Claus

AU - Leib-Mösch, Christine

AU - Salmons, Brian

AU - Günzburg, Walter H.

AU - Baumann, Gaby

AU - Höfler, Heinz

AU - Erfle, Volker

AU - Atkinson, Michael J.

N1 - Funding Information: We are grateful to W. Gimbel, Institute of Molecular Virology for helpful discussions in establishing the RELAP-PCR technique, Rainer H. Dunkl for assistance with the cell culture. We also thank the AG BioDV of the GSF-research center, especially U. Linzner and T. Werner for providing oligonucleotide primers. T. Mietke, Institute of Microbiology, Technical University Munich, kindly provided a GR-mouse liver for DNA isolation. Supported by grants from the Commission of the European Commission CT95-0008b (M.J.A) and DFG (Deutsche Forschungs Gemeinschaft) Ca 165/1-1 (C.C.).

PY - 1998/4

Y1 - 1998/4

N2 - The de novo integration of retroviral genomes within the mammalian genome is believed to contribute to the tumorigenic process. Integration may result in the disruption or inappropriate transcription of key regulatory genes. We describe the application of an arbitrarily primed PCR method for the mapping and cloning of genomic integration sites of the mouse mammary tumor virus (MMTV). We have amplified DNA sequences between a selected retroviral MMTV-LTR and random sites in the 3' flanking DNA. Using this technique we were able to visualize several proviral integration sites present in a MMTV-induced mammary tumor derived cell line that were absent from the germ line. Cloning and sequencing of the PCR product corresponding to one site established its identification as an unique 3' flanking sequence. Copyright (C) 1998 Elsevier Science B.V.

AB - The de novo integration of retroviral genomes within the mammalian genome is believed to contribute to the tumorigenic process. Integration may result in the disruption or inappropriate transcription of key regulatory genes. We describe the application of an arbitrarily primed PCR method for the mapping and cloning of genomic integration sites of the mouse mammary tumor virus (MMTV). We have amplified DNA sequences between a selected retroviral MMTV-LTR and random sites in the 3' flanking DNA. Using this technique we were able to visualize several proviral integration sites present in a MMTV-induced mammary tumor derived cell line that were absent from the germ line. Cloning and sequencing of the PCR product corresponding to one site established its identification as an unique 3' flanking sequence. Copyright (C) 1998 Elsevier Science B.V.

KW - Insertional mutagenesis

KW - Mouse mammary tumor virus

KW - Polymerase chain reaction

KW - Retroviral LTR arbitrarily primed PCR (RELAP-PCR)

KW - Retroviral integration site

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DO - 10.1016/S0168-1702(98)00002-1

M3 - Article

C2 - 9696128

AN - SCOPUS:0032052724

SN - 0168-1702

VL - 54

SP - 207

EP - 215

JO - Virus Research

JF - Virus Research

IS - 2

ER -

Mapping of a mouse mammary tumor virus integration site by retroviral LTR - Arbitrary polymerase chain reaction

Abstract

Keywords

UN SDGs

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