Magnetic nanoparticle formulations for DNA and siRNA delivery

Olga Mykhaylyk, Dialekti Vlaskou, Nittaya Tresilwised, Pimolpan Pithayanukul, Winfrid Möller, Christian Plank

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

Newly synthesized magnetic nanomaterials possess high DNA binding capacity either itself or in the presence of a positively charged lipid-based Metafectene™ reagent or branched polyethylene imine 25 kDa. Polyethylene imine (PEI)-modified nanomaterials are able to deliver nucleic acids in cell culture in duplexes. Magnetofection with triplexes of nanomaterials results in higher transduction efficiencies compared to optimal PEI or Metafectene formulations. 90% transient down-regulation of the target protein in HeLa-green fluorescence protein cells was achieved at short interfering RNA concentrations as low as 8 nM with a formulation of PEI-modified nanoparticles.

Original languageEnglish
Pages (from-to)275-281
Number of pages7
JournalJournal of Magnetism and Magnetic Materials
Volume311
Issue number1 SPEC. ISS.
DOIs
StatePublished - Apr 2007

Keywords

  • DNA and siRNA
  • Magnetic nanomaterial
  • Magnetofection
  • Non-uniform magnetic field
  • Nucleic acid delivery

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