Magnetic microbubbles: Magnetically targeted and ultrasound-triggered vectors for gene delivery in vitro

Dialechti Vlaskou, Pallab Pradhan, Christian Bergemann, Alexander L. Klibanov, Karin Hensel, Georg Schmitz, Christian Plank, Olga Mykhaylyk

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

15 Scopus citations

Abstract

Based on the concept of magnetofection, we prepared lipid shell microbubbles loaded with highly positively charged iron oxide magnetic nanoparticles through electrostatic and matrix affinity interactions. These magnetic microbubbles showed strong ultrasound contrast. When the magnetic microbubbles were mixed with plasmid DNA encoding a reporter gene, gene delivery to HeLa cells was achieved only when ultrasound was applied. Gene transfer efficiency strongly depended on the application of a gradient magnetic field. Treatment of HeLa cells with the microbubbles and ultrasound resulted in strong concentration-dependent cytotoxic effects, whereas ultrasound alone, lipid microbubbles alone, magnetic nanoparticles or magnetic microbubbles alone did not significantly affect cell viability. These magnetic microbubbles could be used as magnetically targeted diagnostic agents for real-time ultrasound imaging or for cancer therapy, therapy of vascular thrombosis and gene therapy.

Original languageEnglish
Title of host publication8th International Conference on the Scientific and Clinical Applications of Magnetic Carriers
Pages485-494
Number of pages10
DOIs
StatePublished - 2010
Externally publishedYes
Event8th International Conference on the Scientific and Clinical Applications of Magnetic Carriers - Rostock, Germany
Duration: 25 May 201029 May 2010

Publication series

NameAIP Conference Proceedings
Volume1311
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference8th International Conference on the Scientific and Clinical Applications of Magnetic Carriers
Country/TerritoryGermany
CityRostock
Period25/05/1029/05/10

Keywords

  • gene delivery
  • magnetic microbubbles
  • magnetofection
  • sonoporation
  • ultrasound imaging

Fingerprint

Dive into the research topics of 'Magnetic microbubbles: Magnetically targeted and ultrasound-triggered vectors for gene delivery in vitro'. Together they form a unique fingerprint.

Cite this