Magnetic capture-hybridization method for purification and probing of mRNA for neutral protease of Bacillus cereus

Hans Jürgen Bach; Anton Hartmann; Jack T. Trevors; Jean Charles Munch

doi:10.1016/S0167-7012(99)00066-4

Magnetic capture-hybridization method for purification and probing of mRNA for neutral protease of Bacillus cereus

Hans Jürgen Bach
, Anton Hartmann
, Jack T. Trevors
, Jean Charles Munch

Helmholtz Zentrum München German Research Center for Environmental Health
University of Guelph

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A magnetic capture-hybridization method was assessed for the isolation of prokaryotic mRNA for the neutral protease of B. cereus from liquid culture. A biotin-labeled specific probe was hybridized to the mRNA transcripts and subsequently captured by streptavidin-coated paramagnetic beads. mRNA was detected by dot-blot hybridization with a ds DIG-labeled DNA-probe. The magnetic capture hybridization is a rapid and simple method and has a promising potential for gene expression studies in complex samples. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	187-192
Number of pages	6
Journal	Journal of Microbiological Methods
Volume	37
Issue number	2
DOIs	https://doi.org/10.1016/S0167-7012(99)00066-4
State	Published - Aug 1999
Externally published	Yes

Keywords

Bacillus cereus
Magnetic capture-hybridization
Neutral protease
Prokaryotic mRNA

Access to Document

10.1016/S0167-7012(99)00066-4

Cite this

@article{48a6159ced844d24bd1ddcb8bcae6369,

title = "Magnetic capture-hybridization method for purification and probing of mRNA for neutral protease of Bacillus cereus",

abstract = "A magnetic capture-hybridization method was assessed for the isolation of prokaryotic mRNA for the neutral protease of B. cereus from liquid culture. A biotin-labeled specific probe was hybridized to the mRNA transcripts and subsequently captured by streptavidin-coated paramagnetic beads. mRNA was detected by dot-blot hybridization with a ds DIG-labeled DNA-probe. The magnetic capture hybridization is a rapid and simple method and has a promising potential for gene expression studies in complex samples. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Bacillus cereus, Magnetic capture-hybridization, Neutral protease, Prokaryotic mRNA",

author = "Bach, \{Hans J{\"u}rgen\} and Anton Hartmann and Trevors, \{Jack T.\} and Munch, \{Jean Charles\}",

note = "Funding Information: I would like to thank to Dr. D. Wenderoth, Dr. R. Hensik, Dr. G. M{\"o}ller and B. Fellerhoff for very helpful advice on mRNA handling. This study was supported by a grant of the Deutsche Forschungsgemeinschaft (Germany). Research by J.T.T. is supported by a NSERC (Canada) operating grant. ",

year = "1999",

month = aug,

doi = "10.1016/S0167-7012(99)00066-4",

language = "English",

volume = "37",

pages = "187--192",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Magnetic capture-hybridization method for purification and probing of mRNA for neutral protease of Bacillus cereus

AU - Bach, Hans Jürgen

AU - Hartmann, Anton

AU - Trevors, Jack T.

AU - Munch, Jean Charles

N1 - Funding Information: I would like to thank to Dr. D. Wenderoth, Dr. R. Hensik, Dr. G. Möller and B. Fellerhoff for very helpful advice on mRNA handling. This study was supported by a grant of the Deutsche Forschungsgemeinschaft (Germany). Research by J.T.T. is supported by a NSERC (Canada) operating grant.

PY - 1999/8

Y1 - 1999/8

N2 - A magnetic capture-hybridization method was assessed for the isolation of prokaryotic mRNA for the neutral protease of B. cereus from liquid culture. A biotin-labeled specific probe was hybridized to the mRNA transcripts and subsequently captured by streptavidin-coated paramagnetic beads. mRNA was detected by dot-blot hybridization with a ds DIG-labeled DNA-probe. The magnetic capture hybridization is a rapid and simple method and has a promising potential for gene expression studies in complex samples. Copyright (C) 1999 Elsevier Science B.V.

AB - A magnetic capture-hybridization method was assessed for the isolation of prokaryotic mRNA for the neutral protease of B. cereus from liquid culture. A biotin-labeled specific probe was hybridized to the mRNA transcripts and subsequently captured by streptavidin-coated paramagnetic beads. mRNA was detected by dot-blot hybridization with a ds DIG-labeled DNA-probe. The magnetic capture hybridization is a rapid and simple method and has a promising potential for gene expression studies in complex samples. Copyright (C) 1999 Elsevier Science B.V.

KW - Bacillus cereus

KW - Magnetic capture-hybridization

KW - Neutral protease

KW - Prokaryotic mRNA

UR - https://www.scopus.com/pages/publications/0033041422

U2 - 10.1016/S0167-7012(99)00066-4

DO - 10.1016/S0167-7012(99)00066-4

M3 - Article

C2 - 10445318

AN - SCOPUS:0033041422

SN - 0167-7012

VL - 37

SP - 187

EP - 192

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 2

ER -

Magnetic capture-hybridization method for purification and probing of mRNA for neutral protease of Bacillus cereus

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this