Macrophage cholesterol efflux correlates with lipoprotein subclass distribution and risk of obstructive coronary artery disease in patients undergoing coronary angiography

Patrick Linsel-Nitschke, Henning Jansen, Zouhair Aherrarhou, Stefanie Belz, Björn Mayer, Wolfgang Lieb, Fritz Huber, Werner Kremer, Hans Robert Kalbitzer, Jeanette Erdmann, Heribert Schunkert

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Background. Studies in patients with low HDL have suggested that impaired cellular cholesterol efflux is a heritable phenotype increasing atherosclerosis risk. Less is known about the association of macrophage cholesterol efflux with lipid profiles and CAD risk in normolipidemic subjects. We have therefore measured macrophage cholesterol efflux in142 normolipidemic subjects undergoing coronary angiography. Methods. Monocytes isolated from blood samples of patients scheduled for cardiac catheterization were differentiated into macrophages over seven days. Isotopic cholesterol efflux to exogenously added apolipoprotein A-I and HDL2 was measured. Quantitative cholesterol efflux from macrophages was correlated with lipoprotein subclass distribution in plasma from the same individuals measured by NMR-spectroscopy of lipids and with the extent of coronary artery disease seen on coronary angiography. Results. Macrophage cholesterol efflux was positively correlated with particle concentration of smaller HDL and LDL particles but not with total plasma concentrations of HDL or LDL-cholesterol. We observed an inverse relationship between macrophage cholesterol efflux and the concntration of larger and triglyceride rich particles (VLDL, chylomicrons). Subjects with significant stenosis on coronary angiography had lower cholesterol efflux from macrophages compared to individuals without significant stenosis (adjusted p = 0.02). Conclusion. Macrophage cholesterol efflux is inversely correlated with lipoprotein particle size and risk of CAD.

Original languageEnglish
Article number14
JournalLipids in Health and Disease
Volume8
DOIs
StatePublished - 2009
Externally publishedYes

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