Mössbauer spectroscopy in the energy and in the time domain, a crucial tool for the investigation of protein dynamics

Alexandra Huenges; Klaus Achterhold; Fritz G. Parak

doi:10.1023/A:1025405805908

Mössbauer spectroscopy in the energy and in the time domain, a crucial tool for the investigation of protein dynamics

Alexandra Huenges
, Klaus Achterhold
, Fritz G. Parak

Chair of Biomedical Physics

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

New experiments on MbCO crystals and MbCO in a glycerol/water solution are described. Phonon-assisted Mössbauer effect reveals solid state like harmonic vibrations and is used to determine the phonon density of states spectrum at the iron center of myoglobin. Above 200 K, a new type of fluctuations arises which is essential for protein function. The experiments can be explained by a two state model. In the "rigid" state, harmonic vibrations are present in the full temperature regime. Above 200 K a "flexible" state can be reached. The quasi-diffusive motions in this state can be described by an overdamped Brownian oscillator with only one frequency. The Boltzmann probability to reach the flexible state is used. The influence of the solvent surrounding the protein is investigated. The dynamical transition temperature of the protein is compared with a glass transition.

Original language	English
Pages (from-to)	209-222
Number of pages	14
Journal	Hyperfine Interactions
Volume	144-145
Issue number	1-4
DOIs	https://doi.org/10.1023/A:1025405805908
State	Published - 2002

Keywords

Brownian oscillator
Conformational transition
Density of phonon states
Myoglobin

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10.1023/A:1025405805908

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title = "M{\"o}ssbauer spectroscopy in the energy and in the time domain, a crucial tool for the investigation of protein dynamics",

abstract = "New experiments on MbCO crystals and MbCO in a glycerol/water solution are described. Phonon-assisted M{\"o}ssbauer effect reveals solid state like harmonic vibrations and is used to determine the phonon density of states spectrum at the iron center of myoglobin. Above 200 K, a new type of fluctuations arises which is essential for protein function. The experiments can be explained by a two state model. In the {"}rigid{"} state, harmonic vibrations are present in the full temperature regime. Above 200 K a {"}flexible{"} state can be reached. The quasi-diffusive motions in this state can be described by an overdamped Brownian oscillator with only one frequency. The Boltzmann probability to reach the flexible state is used. The influence of the solvent surrounding the protein is investigated. The dynamical transition temperature of the protein is compared with a glass transition.",

keywords = "Brownian oscillator, Conformational transition, Density of phonon states, Myoglobin",

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doi = "10.1023/A:1025405805908",

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TY - JOUR

T1 - Mössbauer spectroscopy in the energy and in the time domain, a crucial tool for the investigation of protein dynamics

AU - Huenges, Alexandra

AU - Achterhold, Klaus

AU - Parak, Fritz G.

PY - 2002

Y1 - 2002

N2 - New experiments on MbCO crystals and MbCO in a glycerol/water solution are described. Phonon-assisted Mössbauer effect reveals solid state like harmonic vibrations and is used to determine the phonon density of states spectrum at the iron center of myoglobin. Above 200 K, a new type of fluctuations arises which is essential for protein function. The experiments can be explained by a two state model. In the "rigid" state, harmonic vibrations are present in the full temperature regime. Above 200 K a "flexible" state can be reached. The quasi-diffusive motions in this state can be described by an overdamped Brownian oscillator with only one frequency. The Boltzmann probability to reach the flexible state is used. The influence of the solvent surrounding the protein is investigated. The dynamical transition temperature of the protein is compared with a glass transition.

AB - New experiments on MbCO crystals and MbCO in a glycerol/water solution are described. Phonon-assisted Mössbauer effect reveals solid state like harmonic vibrations and is used to determine the phonon density of states spectrum at the iron center of myoglobin. Above 200 K, a new type of fluctuations arises which is essential for protein function. The experiments can be explained by a two state model. In the "rigid" state, harmonic vibrations are present in the full temperature regime. Above 200 K a "flexible" state can be reached. The quasi-diffusive motions in this state can be described by an overdamped Brownian oscillator with only one frequency. The Boltzmann probability to reach the flexible state is used. The influence of the solvent surrounding the protein is investigated. The dynamical transition temperature of the protein is compared with a glass transition.

KW - Brownian oscillator

KW - Conformational transition

KW - Density of phonon states

KW - Myoglobin

UR - https://www.scopus.com/pages/publications/0141738315

U2 - 10.1023/A:1025405805908

DO - 10.1023/A:1025405805908

M3 - Article

AN - SCOPUS:0141738315

SN - 0304-3843

VL - 144-145

SP - 209

EP - 222

JO - Hyperfine Interactions

JF - Hyperfine Interactions

IS - 1-4

ER -

Mössbauer spectroscopy in the energy and in the time domain, a crucial tool for the investigation of protein dynamics

Abstract

Keywords

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