TY - JOUR
T1 - Lysinibacillus irui sp. nov., isolated from Iru, fermented African locust beans
AU - Akintayo, Stephen Olusanmi
AU - Neumann, Bernd
AU - Fischer, Martin
AU - Henkel, Marius
AU - Lilge, Lars
AU - Hausmann, Rudolf
N1 - Publisher Copyright:
© 2023 The Authors.
PY - 2023/11/1
Y1 - 2023/11/1
N2 - A Gram-positive, motile, aerobic, rod-shaped, endospore-forming strain designated IRB4-01T was isolated from fermented African locust beans (Iru) obtained from Bodija market in the city of Ibadan, southwestern Nigeria, during a screening process from food-related sources. IRB4-01T grew at 10–50 °C (optimum, 35–37 °C), pH 6–10 (optimum, pH 7) and in 0–6 % NaCl (optimum, 1–3 %). Phylogenetic analyses based on 16S rRNA and combined short-and long-read genome sequencing revealed that IRB4-01T is closely related to Lysinibacillus cavernae SYSU K30005T and Lysinibacillus boronitolerans 10aT. The cell-wall peptidoglycan type was A4α (Lys–Asp), containing the diagnostic diamino acid lysine. The major polar lipids in strain IRB4-01T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid, while the predominant menaquinone was MK-7. The major cellular fatty acids were iso-C15: 0, anteiso-C15: 0 and iso-C16: 0. Genomic DNA G+C content was 37.4 mol%, while the digital DNA–DNA hybridization revealed 33.6 and 32.3 % relatedness to L. cavernae SYSU K30005T and L. boronitolerans 10aT, respectively. Based on phenotypic, physiological and chemotaxonomic characteristics, as well as genome comparisons, strain IRB4-01T represents a novel species of the genus Lysinibacillus, for which the name Lysini-bacillus irui sp. nov. is proposed. The type strain is IRB4-01T (NCIMB 15452T=LMG 32887T). Hybrid genome data are provided on the NCBI database using the Bioproject number PRJNA906010 and accession numbers CP113527 and CP113528. Additionally, a representative 16S rRNA sequence is available with the GenBank accession number OQ566940.
AB - A Gram-positive, motile, aerobic, rod-shaped, endospore-forming strain designated IRB4-01T was isolated from fermented African locust beans (Iru) obtained from Bodija market in the city of Ibadan, southwestern Nigeria, during a screening process from food-related sources. IRB4-01T grew at 10–50 °C (optimum, 35–37 °C), pH 6–10 (optimum, pH 7) and in 0–6 % NaCl (optimum, 1–3 %). Phylogenetic analyses based on 16S rRNA and combined short-and long-read genome sequencing revealed that IRB4-01T is closely related to Lysinibacillus cavernae SYSU K30005T and Lysinibacillus boronitolerans 10aT. The cell-wall peptidoglycan type was A4α (Lys–Asp), containing the diagnostic diamino acid lysine. The major polar lipids in strain IRB4-01T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid, while the predominant menaquinone was MK-7. The major cellular fatty acids were iso-C15: 0, anteiso-C15: 0 and iso-C16: 0. Genomic DNA G+C content was 37.4 mol%, while the digital DNA–DNA hybridization revealed 33.6 and 32.3 % relatedness to L. cavernae SYSU K30005T and L. boronitolerans 10aT, respectively. Based on phenotypic, physiological and chemotaxonomic characteristics, as well as genome comparisons, strain IRB4-01T represents a novel species of the genus Lysinibacillus, for which the name Lysini-bacillus irui sp. nov. is proposed. The type strain is IRB4-01T (NCIMB 15452T=LMG 32887T). Hybrid genome data are provided on the NCBI database using the Bioproject number PRJNA906010 and accession numbers CP113527 and CP113528. Additionally, a representative 16S rRNA sequence is available with the GenBank accession number OQ566940.
KW - Lysinibacillus
KW - biotechnology
KW - food
KW - genome analysis
UR - http://www.scopus.com/inward/record.url?scp=85176101625&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.006167
DO - 10.1099/ijsem.0.006167
M3 - Article
AN - SCOPUS:85176101625
SN - 1466-5026
VL - 73
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 11
M1 - 006167
ER -