Lysate Preparation for Reverse Phase Protein Arrays

Karl Friedrich Becker

doi:10.1007/978-981-32-9755-5_2

Lysate Preparation for Reverse Phase Protein Arrays

Karl Friedrich Becker

Medicine and Health

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Scopus citations

Abstract

Protein lysates from a variety of sample materials, e.g., cell lines, serum, frozen tissues, formalin-fixed and paraffin-embedded (FFPE) tissues, and fine needle aspirates, are compatible with the analysis using reverse phase protein arrays (RPPAs). Due to this diversity of input material, lysate preparation is one of the critical steps for obtaining reliable RPPA results. The challenges include, but are not limited to, achieving complete solubilization, avoiding chemical or enzymatic protein modifications, and degradation. In this chapter, preparations of lysates for RPPA analysis are described, focusing on human tissue samples. Special emphasis is given to recently published ISO International Standards for the preanalytical phase.

Original language	English
Title of host publication	Advances in Experimental Medicine and Biology
Publisher	Springer
Pages	21-30
Number of pages	10
DOIs	https://doi.org/10.1007/978-981-32-9755-5_2
State	Published - 2019

Publication series

Name	Advances in Experimental Medicine and Biology
Volume	1188
ISSN (Print)	0065-2598
ISSN (Electronic)	2214-8019

Keywords

FFPE
Frozen
ISO
International standard
Protein isolation
Protein lysate
RPPA
Tissue

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10.1007/978-981-32-9755-5_2

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title = "Lysate Preparation for Reverse Phase Protein Arrays",

abstract = "Protein lysates from a variety of sample materials, e.g., cell lines, serum, frozen tissues, formalin-fixed and paraffin-embedded (FFPE) tissues, and fine needle aspirates, are compatible with the analysis using reverse phase protein arrays (RPPAs). Due to this diversity of input material, lysate preparation is one of the critical steps for obtaining reliable RPPA results. The challenges include, but are not limited to, achieving complete solubilization, avoiding chemical or enzymatic protein modifications, and degradation. In this chapter, preparations of lysates for RPPA analysis are described, focusing on human tissue samples. Special emphasis is given to recently published ISO International Standards for the preanalytical phase.",

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AB - Protein lysates from a variety of sample materials, e.g., cell lines, serum, frozen tissues, formalin-fixed and paraffin-embedded (FFPE) tissues, and fine needle aspirates, are compatible with the analysis using reverse phase protein arrays (RPPAs). Due to this diversity of input material, lysate preparation is one of the critical steps for obtaining reliable RPPA results. The challenges include, but are not limited to, achieving complete solubilization, avoiding chemical or enzymatic protein modifications, and degradation. In this chapter, preparations of lysates for RPPA analysis are described, focusing on human tissue samples. Special emphasis is given to recently published ISO International Standards for the preanalytical phase.

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KW - ISO

KW - International standard

KW - Protein isolation

KW - Protein lysate

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KW - Tissue

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BT - Advances in Experimental Medicine and Biology

PB - Springer

ER -

Lysate Preparation for Reverse Phase Protein Arrays

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