Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription

Giacomo Cossa; Isabelle Roeschert; Florian Prinz; Apoorva Baluapuri; Raphael Silveira Vidal; Christina Schülein-Völk; Yun Chien Chang; Carsten Patrick Ade; Guido Mastrobuoni; Cyrille Girard; Lars Wortmann; Susanne Walz; Reinhard Lührmann; Stefan Kempa; Bernhard Kuster; Elmar Wolf; Dominik Mumberg; Martin Eilers

doi:10.1016/j.molcel.2020.01.008

Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription

Giacomo Cossa
, Isabelle Roeschert
, Florian Prinz
, Apoorva Baluapuri
, Raphael Silveira Vidal
, Christina Schülein-Völk
, Yun Chien Chang
, Carsten Patrick Ade
, Guido Mastrobuoni
, Cyrille Girard
, Lars Wortmann
, Susanne Walz
, Reinhard Lührmann
, Stefan Kempa
, Bernhard Kuster
, Elmar Wolf
, Dominik Mumberg
, Martin Eilers

Chair of Bioanalytics

University of Würzburg
Therapeutic Research Group Women's Healthcare Bayer Schering Pharma AG
Technical University of Munich
Max Delbrück Center for Molecular Medicine
Max Planck Institute for Biophysical Chemistry

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Tumors displaying high MYC levels depend on the NUAK1 kinase. Cossa et al. show that NUAK1 binds protein phosphatase 1 (PP1) and its regulatory subunit PNUTS. They show that this complex associates with the splicing machinery and provides a feedback control of transcription that can be overridden by deregulated MYC.

Original language	English
Pages (from-to)	1322-1339.e11
Journal	Molecular Cell
Volume	77
Issue number	6
DOIs	https://doi.org/10.1016/j.molcel.2020.01.008
State	Published - 19 Mar 2020

Keywords

ARK5
MYC
NUAK1
PNUTS
PP1
Protein Phosphatase 1
Spliceosome

Access to Document

10.1016/j.molcel.2020.01.008

Cite this

Cossa, G., Roeschert, I., Prinz, F., Baluapuri, A., Silveira Vidal, R., Schülein-Völk, C., Chang, Y. C., Ade, C. P., Mastrobuoni, G., Girard, C., Wortmann, L., Walz, S., Lührmann, R., Kempa, S., Kuster, B., Wolf, E., Mumberg, D., & Eilers, M. (2020). Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription. Molecular Cell, 77(6), 1322-1339.e11. https://doi.org/10.1016/j.molcel.2020.01.008

@article{4fc738e6dc764c75b726afeff6c15a92,

title = "Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription",

abstract = "Tumors displaying high MYC levels depend on the NUAK1 kinase. Cossa et al. show that NUAK1 binds protein phosphatase 1 (PP1) and its regulatory subunit PNUTS. They show that this complex associates with the splicing machinery and provides a feedback control of transcription that can be overridden by deregulated MYC.",

keywords = "ARK5, MYC, NUAK1, PNUTS, PP1, Protein Phosphatase 1, Spliceosome",

author = "Giacomo Cossa and Isabelle Roeschert and Florian Prinz and Apoorva Baluapuri and \{Silveira Vidal\}, Raphael and Christina Sch{\"u}lein-V{\"o}lk and Chang, \{Yun Chien\} and Ade, \{Carsten Patrick\} and Guido Mastrobuoni and Cyrille Girard and Lars Wortmann and Susanne Walz and Reinhard L{\"u}hrmann and Stefan Kempa and Bernhard Kuster and Elmar Wolf and Dominik Mumberg and Martin Eilers",

note = "Publisher Copyright: {\textcopyright} 2020 The Authors",

year = "2020",

month = mar,

day = "19",

doi = "10.1016/j.molcel.2020.01.008",

language = "English",

volume = "77",

pages = "1322--1339.e11",

journal = "Molecular Cell",

issn = "1097-2765",

publisher = "Cell Press",

number = "6",

}

Cossa, G, Roeschert, I, Prinz, F, Baluapuri, A, Silveira Vidal, R, Schülein-Völk, C, Chang, YC, Ade, CP, Mastrobuoni, G, Girard, C, Wortmann, L, Walz, S, Lührmann, R, Kempa, S, Kuster, B, Wolf, E, Mumberg, D & Eilers, M 2020, 'Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription', Molecular Cell, vol. 77, no. 6, pp. 1322-1339.e11. https://doi.org/10.1016/j.molcel.2020.01.008

TY - JOUR

T1 - Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription

AU - Cossa, Giacomo

AU - Roeschert, Isabelle

AU - Prinz, Florian

AU - Baluapuri, Apoorva

AU - Silveira Vidal, Raphael

AU - Schülein-Völk, Christina

AU - Chang, Yun Chien

AU - Ade, Carsten Patrick

AU - Mastrobuoni, Guido

AU - Girard, Cyrille

AU - Wortmann, Lars

AU - Walz, Susanne

AU - Lührmann, Reinhard

AU - Kempa, Stefan

AU - Kuster, Bernhard

AU - Wolf, Elmar

AU - Mumberg, Dominik

AU - Eilers, Martin

PY - 2020/3/19

Y1 - 2020/3/19

N2 - Tumors displaying high MYC levels depend on the NUAK1 kinase. Cossa et al. show that NUAK1 binds protein phosphatase 1 (PP1) and its regulatory subunit PNUTS. They show that this complex associates with the splicing machinery and provides a feedback control of transcription that can be overridden by deregulated MYC.

AB - Tumors displaying high MYC levels depend on the NUAK1 kinase. Cossa et al. show that NUAK1 binds protein phosphatase 1 (PP1) and its regulatory subunit PNUTS. They show that this complex associates with the splicing machinery and provides a feedback control of transcription that can be overridden by deregulated MYC.

KW - ARK5

KW - MYC

KW - NUAK1

KW - PNUTS

KW - PP1

KW - Protein Phosphatase 1

KW - Spliceosome

UR - https://www.scopus.com/pages/publications/85079816556

U2 - 10.1016/j.molcel.2020.01.008

DO - 10.1016/j.molcel.2020.01.008

M3 - Article

C2 - 32006464

AN - SCOPUS:85079816556

SN - 1097-2765

VL - 77

SP - 1322-1339.e11

JO - Molecular Cell

JF - Molecular Cell

IS - 6

ER -

Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this