Localization of transforming growth factor-β-expressing cells and comparison with major extracellular components in aural cholesteatoma

Stephan Lang, Volker Schilling, Brigitte Mack, Barbara Wollenberg, Andreas Nerlich

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Transforming growth factor-β (TGF-β) plays an important role in the regulation of extracellular matrix (ECM) deposition by stimulating the synthesis of individual matrix proteins like tenascin and fibronectin. Cholesteatoma shows significant changes in the ECM, supporting the view of a disturbed cell-matrix interaction. The purpose of our present study was to evaluate the distribution of TGF- in comparison to the deposition of tenascin, fibronectin, and collagen as major components of the ECM in cholesteatoma (n = 12) by means of histochemistry and immunohistochemistry. We found TGF-β in lymphocytes and fibrohistiocytes in the stroma of 7 cholesteatomas. In corresponding sections, a marked expression of tenascin and fibronectin was seen manifesting as a continuous band along the epidermal-stromal junction, extending to the deeper stroma. In addition, in those cases of TGF-β expression, beginning collagen fibril formation was seen in adjacent deeper stroma layers, indicating beginning stromal fibrosis. These results suggest that TGF-β may be involved in the stimulation of the synthesis of tenascin, fibronectin, and collagen. Furthermore, the enhanced expression of tenascin and fibronectin provides evidence for a deregulated cell-matrix interaction in cholesteatoma associated with the enhanced proliferative process of cholesteatoma formation.

Original languageEnglish
Pages (from-to)669-673
Number of pages5
JournalAnnals of Otology, Rhinology and Laryngology
Volume106
Issue number8
DOIs
StatePublished - 1997
Externally publishedYes

Keywords

  • Cholesteatoma
  • TGF-β
  • Transforming growth factor-β

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