Localization of somatostatin mRNA in the gut, pancreas and thyroid gland of the rat using antisense RNA probes for in situ hybridization.

H. Höfler; H. Childers; M. R. Montminy; R. H. Goodman; R. M. Lechan; R. A. DeLellis; A. S. Tischler; H. J. Wolfe

Localization of somatostatin mRNA in the gut, pancreas and thyroid gland of the rat using antisense RNA probes for in situ hybridization.

H. Höfler, H. Childers, M. R. Montminy, R. H. Goodman, R. M. Lechan, R. A. DeLellis, A. S. Tischler, H. J. Wolfe

TUM Emeriti of Excellence

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Abstract

A method for the localization of rat pre-prosomatostatin mRNA by in situ hybridization with 32P- and 3H-labeled antisense RNA probes is reported. Somatostatin mRNA was detected in endocrine cells of the rat gut mucosa, pancreas and thyroid in a distribution identical to immunoreactive somatostatin. In addition, in situ hybridization allowed localization of reactive neurons in the submucous and myenteric plexus of the gut, sites which are variably positive or negative for immunoreactive somatostatin even after colchicine treatment. These studies indicate that in situ hybridization is more sensitive than immunohistochemistry in some instances for demonstration of somatostatin in gene expression.

Original language	English
Pages (from-to)	101-105
Number of pages	5
Journal	Acta histochemica. Supplementband
Volume	34
State	Published - 1987

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title = "Localization of somatostatin mRNA in the gut, pancreas and thyroid gland of the rat using antisense RNA probes for in situ hybridization.",

abstract = "A method for the localization of rat pre-prosomatostatin mRNA by in situ hybridization with 32P- and 3H-labeled antisense RNA probes is reported. Somatostatin mRNA was detected in endocrine cells of the rat gut mucosa, pancreas and thyroid in a distribution identical to immunoreactive somatostatin. In addition, in situ hybridization allowed localization of reactive neurons in the submucous and myenteric plexus of the gut, sites which are variably positive or negative for immunoreactive somatostatin even after colchicine treatment. These studies indicate that in situ hybridization is more sensitive than immunohistochemistry in some instances for demonstration of somatostatin in gene expression.",

author = "H. H{\"o}fler and H. Childers and Montminy, {M. R.} and Goodman, {R. H.} and Lechan, {R. M.} and DeLellis, {R. A.} and Tischler, {A. S.} and Wolfe, {H. J.}",

year = "1987",

language = "English",

volume = "34",

pages = "101--105",

journal = "Acta histochemica. Supplementband",

issn = "0567-7556",

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TY - JOUR

T1 - Localization of somatostatin mRNA in the gut, pancreas and thyroid gland of the rat using antisense RNA probes for in situ hybridization.

AU - Höfler, H.

AU - Childers, H.

AU - Montminy, M. R.

AU - Goodman, R. H.

AU - Lechan, R. M.

AU - DeLellis, R. A.

AU - Tischler, A. S.

AU - Wolfe, H. J.

PY - 1987

Y1 - 1987

N2 - A method for the localization of rat pre-prosomatostatin mRNA by in situ hybridization with 32P- and 3H-labeled antisense RNA probes is reported. Somatostatin mRNA was detected in endocrine cells of the rat gut mucosa, pancreas and thyroid in a distribution identical to immunoreactive somatostatin. In addition, in situ hybridization allowed localization of reactive neurons in the submucous and myenteric plexus of the gut, sites which are variably positive or negative for immunoreactive somatostatin even after colchicine treatment. These studies indicate that in situ hybridization is more sensitive than immunohistochemistry in some instances for demonstration of somatostatin in gene expression.

AB - A method for the localization of rat pre-prosomatostatin mRNA by in situ hybridization with 32P- and 3H-labeled antisense RNA probes is reported. Somatostatin mRNA was detected in endocrine cells of the rat gut mucosa, pancreas and thyroid in a distribution identical to immunoreactive somatostatin. In addition, in situ hybridization allowed localization of reactive neurons in the submucous and myenteric plexus of the gut, sites which are variably positive or negative for immunoreactive somatostatin even after colchicine treatment. These studies indicate that in situ hybridization is more sensitive than immunohistochemistry in some instances for demonstration of somatostatin in gene expression.

UR - http://www.scopus.com/inward/record.url?scp=0023083762&partnerID=8YFLogxK

M3 - Article

C2 - 2437612

AN - SCOPUS:0023083762

SN - 0567-7556

VL - 34

SP - 101

EP - 105

JO - Acta histochemica. Supplementband

JF - Acta histochemica. Supplementband

ER -

Localization of somatostatin mRNA in the gut, pancreas and thyroid gland of the rat using antisense RNA probes for in situ hybridization.

Abstract

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