TY - JOUR
T1 - Local gene targeting and cell positioning using magnetic nanoparticles and magnetic tips
T2 - Comparison of mathematical simulations with experiments
AU - Kilgus, Carsten
AU - Heidsieck, Alexandra
AU - Ottersbach, Annika
AU - Roell, Wilhelm
AU - Trueck, Christina
AU - Fleischmann, Bernd K.
AU - Gleich, Bernhard
AU - Sasse, Philipp
PY - 2012/5
Y1 - 2012/5
N2 - Purpose: Magnetic nanoparticles (MNPs) and magnets can be used to enhance gene transfer or cell attachment but gene or cell delivery to confined areas has not been addressed. We therefore searched for an optimal method to simulate and perform local gene targeting and cell delivery in vitro. Methods: Localized gene transfer or cell positioning was achieved using permanent magnets with newly designed soft iron tips and MNP/lentivirus complexes or MNP-loaded cells, respectively. Their distribution was simulated with a mathematical model calculating magnetic flux density gradients and particle trajectories. Results: Soft iron tips generated strong confined magnetic fields and could be reliably used for local (∼500 μm diameter) gene targeting and positioning of bone marrow cells or cardiomyocytes. The calculated distribution of MNP/lentivirus complexes and MNP-loaded cells concurred very well with the experimental results of local gene expression and cell attachment, respectively. Conclusion: MNP-based gene targeting and cell positioning can be reliably performed in vitro using magnetic soft iron tips, and computer simulations are effective methods to predict and optimize experimental results.
AB - Purpose: Magnetic nanoparticles (MNPs) and magnets can be used to enhance gene transfer or cell attachment but gene or cell delivery to confined areas has not been addressed. We therefore searched for an optimal method to simulate and perform local gene targeting and cell delivery in vitro. Methods: Localized gene transfer or cell positioning was achieved using permanent magnets with newly designed soft iron tips and MNP/lentivirus complexes or MNP-loaded cells, respectively. Their distribution was simulated with a mathematical model calculating magnetic flux density gradients and particle trajectories. Results: Soft iron tips generated strong confined magnetic fields and could be reliably used for local (∼500 μm diameter) gene targeting and positioning of bone marrow cells or cardiomyocytes. The calculated distribution of MNP/lentivirus complexes and MNP-loaded cells concurred very well with the experimental results of local gene expression and cell attachment, respectively. Conclusion: MNP-based gene targeting and cell positioning can be reliably performed in vitro using magnetic soft iron tips, and computer simulations are effective methods to predict and optimize experimental results.
KW - Cell positioning
KW - Gene targeting
KW - Localization
KW - Magnetic nanoparticles
KW - Mathematical simulation
UR - http://www.scopus.com/inward/record.url?scp=84862686007&partnerID=8YFLogxK
U2 - 10.1007/s11095-011-0647-7
DO - 10.1007/s11095-011-0647-7
M3 - Article
C2 - 22207208
AN - SCOPUS:84862686007
SN - 0724-8741
VL - 29
SP - 1380
EP - 1391
JO - Pharmaceutical Research
JF - Pharmaceutical Research
IS - 5
ER -