Loading-Aware Mixing-Efficient Sample Preparation on Programmable Microfluidic Device

Sample preparation, where a certain number of reagents must be mixed in a specific volumetric ratio, is an integral step for various bio-assays. A programmable microfluidic device (PMD) is an advanced flow-based microfluidic biochip (FMB) platform, that considered to be very effective for sample preparation. However, the impact of mixer placement, reagents' distribution, and mixing time on the automation of sample preparation has not yet been investigated. We consider a mixing efficiency model controlled by the number of alternations 'μ' of reagents along the mixing circulation path and propose a loading-aware placement strategy that maximizes the mixing efficiency. We use satisfiability modulo theories (SMT) and propose a one-pass strategy for placing the mixers and the reagents, that successfully enhance the loading and mixing efficiencies.