Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons

J. Tabitha Hees, Angelika B. Harbauer

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to the available methods, their advantages and drawbacks as well as provide a detailed protocol for the detection of RNA transport using the MS2/PP7-split-Venus system for background-free RNA imaging.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages13
StatePublished - 2022
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cultured primary neurons
  • Imaging
  • MS2
  • PP7
  • RNA transport
  • Split-Venus
  • Transient transfection


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