@inbook{8ebf3575f85d451e840f83dc576e3fd4,
title = "Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons",
abstract = "The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to the available methods, their advantages and drawbacks as well as provide a detailed protocol for the detection of RNA transport using the MS2/PP7-split-Venus system for background-free RNA imaging.",
keywords = "Cultured primary neurons, Imaging, MS2, PP7, RNA transport, Split-Venus, Transient transfection",
author = "Hees, {J. Tabitha} and Harbauer, {Angelika B.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1990-2_11",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "225--237",
booktitle = "Methods in Molecular Biology",
}