Lipoprotein lipase as a new tool in steryl ester analysis

Christine Fischer; Wolfgang Höll

doi:10.1007/BF02544390

Lipoprotein lipase as a new tool in steryl ester analysis

Christine Fischer
, Wolfgang Höll

Chair of Botany

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A new method for the quantitative hydrolysis of steryl esters is described. Using lipoprotein lipase (triacylglycero-protein acylhydrolase, EC 3.1.1.34), steryl esters are cleaved to yield free sterols and fatty acids. In comparison to alkaline hydrolysis, the method is more gentle, more accurate and less laborious. Quantification of the hydrolysis products was done with capillary gas chromatography. The practicability of the method is demonstrated for authentic steryl esters and steryl esters as they occur in needles of Scots pine (Pinus sylvestris L.).

Original language	English
Pages (from-to)	292-295
Number of pages	4
Journal	Lipids
Volume	25
Issue number	5
DOIs	https://doi.org/10.1007/BF02544390
State	Published - May 1990

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title = "Lipoprotein lipase as a new tool in steryl ester analysis",

abstract = "A new method for the quantitative hydrolysis of steryl esters is described. Using lipoprotein lipase (triacylglycero-protein acylhydrolase, EC 3.1.1.34), steryl esters are cleaved to yield free sterols and fatty acids. In comparison to alkaline hydrolysis, the method is more gentle, more accurate and less laborious. Quantification of the hydrolysis products was done with capillary gas chromatography. The practicability of the method is demonstrated for authentic steryl esters and steryl esters as they occur in needles of Scots pine (Pinus sylvestris L.).",

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AU - Höll, Wolfgang

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N2 - A new method for the quantitative hydrolysis of steryl esters is described. Using lipoprotein lipase (triacylglycero-protein acylhydrolase, EC 3.1.1.34), steryl esters are cleaved to yield free sterols and fatty acids. In comparison to alkaline hydrolysis, the method is more gentle, more accurate and less laborious. Quantification of the hydrolysis products was done with capillary gas chromatography. The practicability of the method is demonstrated for authentic steryl esters and steryl esters as they occur in needles of Scots pine (Pinus sylvestris L.).

AB - A new method for the quantitative hydrolysis of steryl esters is described. Using lipoprotein lipase (triacylglycero-protein acylhydrolase, EC 3.1.1.34), steryl esters are cleaved to yield free sterols and fatty acids. In comparison to alkaline hydrolysis, the method is more gentle, more accurate and less laborious. Quantification of the hydrolysis products was done with capillary gas chromatography. The practicability of the method is demonstrated for authentic steryl esters and steryl esters as they occur in needles of Scots pine (Pinus sylvestris L.).

UR - https://www.scopus.com/pages/publications/0025277711

U2 - 10.1007/BF02544390

DO - 10.1007/BF02544390

M3 - Article

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SN - 0024-4201

VL - 25

SP - 292

EP - 295

JO - Lipids

JF - Lipids

IS - 5

ER -

Lipoprotein lipase as a new tool in steryl ester analysis

Abstract

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