Left ventricular functional assessment in murine models of ischemic and dilated cardiomyopathy using [18F]FDG-PET: Comparison with cardiac MRI and monitoring erythropoietin therapy

Stefan Brunner, Andrei Todica, Guido Böning, Stefan G. Nekolla, Moritz Wildgruber, Sebastian Lehner, Martina Sauter, Christopher Übleis, Karin Klingel, Paul Cumming, Wolfgang Michael Franz, Marcus Hacker

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Background: We performed an initial evaluation of non-invasive ECG-gated [18F]FDG-positron emission tomography (FDG-PET) for serial measurements of left ventricular volumes and function in murine models of dilated (DCM) and ischemic cardiomyopathy (ICM), and then tested the effect of erythropoietin (EPO) treatment on DCM mice in a preliminary FDG-PET therapy monitoring study. Methods: Mice developed DCM 8 weeks after injection with Coxsackievirus B3 (CVB3), whereas ICM was induced by ligation of the left anterior descending artery. LV volumes (EDV and ESV) and the ejection fraction (LVEF) of DCM, ICM and healthy control mice were measured by FDG-PET and compared with reference standard results obtained with 1.5 T magnetic resonance imaging (MRI). In the subsequent monitoring study, LVEF of DCM mice was evaluated by FDG-PET at baseline, and after 4 weeks of treatment, with EPO or saline. Results: LV volumes and the LVEF as measured by FDG-PET correlated significantly with the MRI results. These correlations were higher in healthy and DCM mice than in ICM mice, in which LVEF measurements were somewhat compromised by absence of FDG uptake in the area of infarction. LV volumes (EDV and ESV) were systematically underestimated by FDG-PET, with net bias such that LVEF measurements in both models of heart disease exceeded by 15% to 20% results obtained by MRI. In our subsequent monitoring study of DCM mice, we found a significant decrease of LVEF in the EPO group, but not in the saline-treated mice. Moreover, LVEF in the EPO and saline mice significantly correlated with histological scores of fibrosis. Conclusions: LVEF estimated by ECG-gated FDG-PET significantly correlated with the reference standard MRI, most notably in healthy mice and mice with DCM. FDG-PET served for longitudinal monitoring of effects of EPO treatment in DCM mice.

Original languageEnglish
Article number43
Pages (from-to)1-9
Number of pages9
JournalEJNMMI Research
Volume2
Issue number1
DOIs
StatePublished - 2012
Externally publishedYes

Keywords

  • Cardiomyopathy
  • Ejection fraction
  • Erythropoietin
  • Magnetic resonance imaging
  • Positron emission tomography

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