Abstract
This study aimed at developing a sensitive LC-MS/MS method for the quantification of sulforaphane (SFN) and indole-3-carbinol metabolites in plasma and urine after dietary intake of regular and selenium-fertilized broccoli using stable isotope dilution analysis. In a three-armed, placebo-controlled, randomized human intervention study with 76 healthy volunteers, 200 g of regular (485 μg of total glucosinolates and <0.01 μg of selenium per gram fresh weight) or selenium-fertilized broccoli (589 μg of total glucosinolates and 0.25 μg of selenium per gram fresh weight) was administered daily for 4 weeks. Glucoraphanin and glucobrassicin metabolites quantified in plasma and urine were SFN-glutathione, SFN-cysteine, SFN-cysteinylglycine, SFN-acetylcysteine, and indole-3-carboxaldehyde, indole-3-carboxylic acid, and ascorbigen, respectively. Dietary intake of selenium-fertilized broccoli increased serum selenium concentration analyzed by means of atomic absorption spectroscopy by up to 25% (p < 0.001), but affected neither glucosinolate concentrations in broccoli nor their metabolite concentrations in plasma and urine compared to regular broccoli.
| Original language | English |
|---|---|
| Pages (from-to) | 8047-8057 |
| Number of pages | 11 |
| Journal | Journal of agricultural and food chemistry |
| Volume | 59 |
| Issue number | 15 |
| DOIs | |
| State | Published - 10 Aug 2011 |
| Externally published | Yes |
Keywords
- LC-MS/MS
- broccoli
- glucobrassicin metabolites
- glucoraphanin metabolites
- glucosinolates
- indole-3-carbinol
- selenium
- sulforaphane
