Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells

Miguel A. Pleitez; Asrar Ali Khan; Alice Soldà; Andriy Chmyrov; Josefine Reber; Francesca Gasparin; Markus R. Seeger; Benedikt Schätz; Stephan Herzig; Marcel Scheideler; Vasilis Ntziachristos

doi:10.1038/s41587-019-0359-9

Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells

Miguel A. Pleitez, Asrar Ali Khan, Alice Soldà, Andriy Chmyrov, Josefine Reber, Francesca Gasparin, Markus R. Seeger, Benedikt Schätz, Stephan Herzig, Marcel Scheideler, Vasilis Ntziachristos

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

We develop mid-infrared optoacoustic microscopy (MiROM) for label-free, bond-selective, live-cell metabolic imaging, enabling spatiotemporal monitoring of carbohydrates, lipids and proteins in cells and tissues. Using acoustic detection of optical absorption, MiROM converts mid-infrared sensing into a positive-contrast imaging modality with negligible photodamage and high sensitivity. We use MiROM to observe changes in intrinsic carbohydrate distribution from a diffusive spatial pattern to tight co-localization with lipid droplets during adipogenesis.

Original language	English
Pages (from-to)	293-296
Number of pages	4
Journal	Nature Biotechnology
Volume	38
Issue number	3
DOIs	https://doi.org/10.1038/s41587-019-0359-9
State	Published - 1 Mar 2020

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abstract = "We develop mid-infrared optoacoustic microscopy (MiROM) for label-free, bond-selective, live-cell metabolic imaging, enabling spatiotemporal monitoring of carbohydrates, lipids and proteins in cells and tissues. Using acoustic detection of optical absorption, MiROM converts mid-infrared sensing into a positive-contrast imaging modality with negligible photodamage and high sensitivity. We use MiROM to observe changes in intrinsic carbohydrate distribution from a diffusive spatial pattern to tight co-localization with lipid droplets during adipogenesis.",

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AU - Pleitez, Miguel A.

AU - Khan, Asrar Ali

AU - Soldà, Alice

AU - Chmyrov, Andriy

AU - Reber, Josefine

AU - Gasparin, Francesca

AU - Seeger, Markus R.

AU - Schätz, Benedikt

AU - Herzig, Stephan

AU - Scheideler, Marcel

AU - Ntziachristos, Vasilis

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Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells

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