TY - JOUR
T1 - Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells
AU - Pleitez, Miguel A.
AU - Khan, Asrar Ali
AU - Soldà, Alice
AU - Chmyrov, Andriy
AU - Reber, Josefine
AU - Gasparin, Francesca
AU - Seeger, Markus R.
AU - Schätz, Benedikt
AU - Herzig, Stephan
AU - Scheideler, Marcel
AU - Ntziachristos, Vasilis
N1 - Publisher Copyright:
© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - We develop mid-infrared optoacoustic microscopy (MiROM) for label-free, bond-selective, live-cell metabolic imaging, enabling spatiotemporal monitoring of carbohydrates, lipids and proteins in cells and tissues. Using acoustic detection of optical absorption, MiROM converts mid-infrared sensing into a positive-contrast imaging modality with negligible photodamage and high sensitivity. We use MiROM to observe changes in intrinsic carbohydrate distribution from a diffusive spatial pattern to tight co-localization with lipid droplets during adipogenesis.
AB - We develop mid-infrared optoacoustic microscopy (MiROM) for label-free, bond-selective, live-cell metabolic imaging, enabling spatiotemporal monitoring of carbohydrates, lipids and proteins in cells and tissues. Using acoustic detection of optical absorption, MiROM converts mid-infrared sensing into a positive-contrast imaging modality with negligible photodamage and high sensitivity. We use MiROM to observe changes in intrinsic carbohydrate distribution from a diffusive spatial pattern to tight co-localization with lipid droplets during adipogenesis.
UR - http://www.scopus.com/inward/record.url?scp=85076877834&partnerID=8YFLogxK
U2 - 10.1038/s41587-019-0359-9
DO - 10.1038/s41587-019-0359-9
M3 - Article
C2 - 31873214
AN - SCOPUS:85076877834
SN - 1087-0156
VL - 38
SP - 293
EP - 296
JO - Nature Biotechnology
JF - Nature Biotechnology
IS - 3
ER -