KNOCHEMARKKULTUR, ZYTOCHEMIE UND ELEKTRONENMIKROSKOPIE IM AGAR BEI PATIENTEN MIT PRALEUKAMISCHEM SYNDROM UND APLASTISCHER ANAMIE

Translated title of the contribution: Bone marrow culture cytochemistry, and electron microscopy in agar of patients with preleukemia and aplastic anemia

G. Konwalinka, C. Peschel, D. Geissler

Research output: Contribution to journalArticlepeer-review

Abstract

Thirty-seven patients with chronic cytopenia were studied using a CFU-c (colony-forming-unit-culture) assay in agar. On the basis of the growth pattern, 3 types of preleukaemic syndrome (PL) and 2 types of aplastic anaemia were distinguished. Further evaluation of bone-marrow dysfunction was attempted with a combined application of cytochemistry and electron microscopy for the morphologic study of cells proliferating in vitro. Well-defined maturation defects in the growing cells from the bone marrow of patients with PL were demonstrated with cytochemical stainings performed in agar. These results were supported by electron-microscopic findings of Auer-body-like inclusions in 'statu nascendi' in the vacuoles of preleukaemic cells. On the basis of our results, a high-risk group of PL for development of overt leukaemia and a group of patients with a grave prognosis in aplastic anaemia were distinguished. The data obtained are relevant for the clinical diagnosis and prognosis of patients with cytopenias.

Translated title of the contributionBone marrow culture cytochemistry, and electron microscopy in agar of patients with preleukemia and aplastic anemia
Original languageGerman
Pages (from-to)103-108
Number of pages6
JournalActa Medica Austriaca
Volume9
Issue number3
StatePublished - 1982
Externally publishedYes

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