Isothermal assembly of DNA origami structures using denaturing agents

Ralf Jungmann, Tim Liedl, Thomas L. Sobey, William Shih, Friedrich C. Simmel

Research output: Contribution to journalArticlepeer-review

107 Scopus citations

Abstract

DNA origami is one of the most promising recent developments in DNA self-assembly. It allows for the construction of arbitrary nanoscale patterns and objects by folding a long viral scaffold strand using a large number of short "staple" strands. Assembly is usually accomplished by thermal annealing of the DNA molecules in buffer solution. We here demonstrate that both 2D and 3D origami structures can be assembled isothermally by annealing the DNA strands in denaturing buffer, followed by a controlled reduction of denaturant concentration. This opens up origami assembly for the integration of temperature-sensitive components.

Original languageEnglish
Pages (from-to)10062-10063
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number31
DOIs
StatePublished - 6 Aug 2008

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