Isomaltose formation by free and immobilized dextransucrase

Sonja Berensmeier; Hans Joachim Jördening; Klaus Buchholz

doi:10.1080/10242420500491854

Isomaltose formation by free and immobilized dextransucrase

Sonja Berensmeier, Hans Joachim Jördening, Klaus Buchholz

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The acceptor reaction of dextransucrase from Leuconostoc mesenteroides NRRL-B512F with glucose as acceptor is of technical interest for isomaltooligosaccharide (IMOs) synthesis. Different experimental conditions were investigated for free and immobilized enzyme. The data for oligosaccharide formation up to a degree of polymerization 4 were correlated with a model developed earlier, and optimal reaction conditions for immobilized dextransucrase design and application were identified for later continuous application. Furthermore, stability was investigated for free and immobilized enzyme including stabilization by sugars.

Original language	English
Pages (from-to)	280-290
Number of pages	11
Journal	Biocatalysis and Biotransformation
Volume	24
Issue number	4
DOIs	https://doi.org/10.1080/10242420500491854
State	Published - Jul 2006
Externally published	Yes

Keywords

Alginate
Dextransucrase
Fluidized bed
Immobilization
Isomaltooligosaccharide

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10.1080/10242420500491854

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abstract = "The acceptor reaction of dextransucrase from Leuconostoc mesenteroides NRRL-B512F with glucose as acceptor is of technical interest for isomaltooligosaccharide (IMOs) synthesis. Different experimental conditions were investigated for free and immobilized enzyme. The data for oligosaccharide formation up to a degree of polymerization 4 were correlated with a model developed earlier, and optimal reaction conditions for immobilized dextransucrase design and application were identified for later continuous application. Furthermore, stability was investigated for free and immobilized enzyme including stabilization by sugars.",

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author = "Sonja Berensmeier and J{\"o}rdening, {Hans Joachim} and Klaus Buchholz",

note = "Funding Information: This research was supported by the German research foundation (DFG) by means of the Collaborative Research Centre 578 {\textquoteleft}Development of Biotechnological Processes by integrating Genetic and Engineering Methods{\textquoteright}.",

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T1 - Isomaltose formation by free and immobilized dextransucrase

AU - Berensmeier, Sonja

AU - Jördening, Hans Joachim

AU - Buchholz, Klaus

N1 - Funding Information: This research was supported by the German research foundation (DFG) by means of the Collaborative Research Centre 578 ‘Development of Biotechnological Processes by integrating Genetic and Engineering Methods’.

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Y1 - 2006/7

N2 - The acceptor reaction of dextransucrase from Leuconostoc mesenteroides NRRL-B512F with glucose as acceptor is of technical interest for isomaltooligosaccharide (IMOs) synthesis. Different experimental conditions were investigated for free and immobilized enzyme. The data for oligosaccharide formation up to a degree of polymerization 4 were correlated with a model developed earlier, and optimal reaction conditions for immobilized dextransucrase design and application were identified for later continuous application. Furthermore, stability was investigated for free and immobilized enzyme including stabilization by sugars.

AB - The acceptor reaction of dextransucrase from Leuconostoc mesenteroides NRRL-B512F with glucose as acceptor is of technical interest for isomaltooligosaccharide (IMOs) synthesis. Different experimental conditions were investigated for free and immobilized enzyme. The data for oligosaccharide formation up to a degree of polymerization 4 were correlated with a model developed earlier, and optimal reaction conditions for immobilized dextransucrase design and application were identified for later continuous application. Furthermore, stability was investigated for free and immobilized enzyme including stabilization by sugars.

KW - Alginate

KW - Dextransucrase

KW - Fluidized bed

KW - Immobilization

KW - Isomaltooligosaccharide

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EP - 290

JO - Biocatalysis and Biotransformation

JF - Biocatalysis and Biotransformation

IS - 4

ER -

Isomaltose formation by free and immobilized dextransucrase

Abstract

Keywords

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