Isolation of Vibrio alginolyticus from seawater aquaria

Stefan Hörmansdorfer, Helmut Wentges, Karin Neugebaur-Büchler, Johann Bauer

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

The seawater bacterium Vibrio alginolyticus was detected in 5 of 20 water samples from seawater aquaria (from 3 of 5 units) and also from the surface of diseased stony corals. A total of 45 isolates were differentiated biochemically, of which 13 isolates (29 %) proved to be V. alginolyticus. All those strains produced the virulence factors caseinase and lipase, 11 strains amylase and gelatinase. 7 strains showed lecithinase activity and 2 strains produced hemolysins. All examined strains showed a marked toxicity to veto cells proven by the MTT-bioassay, but no toxicity to plant cells with the saline alga Asteromonas gracilis as model. The isolates were mostly resistant to β-lactam antibiotics, macrolides and lincomycin. However, they proved to be susceptible to aminoglycoside- and polypeptide-antibiotics as well as to tetracyclines, chloramphenicol, florfenicol, enrofloxacin and sulfamethoxazol-trimethoprim. The possible participation of this bacterium in the bleaching and dying of stony corals is mentioned as well as its role as human pathogen.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalInternational Journal of Hygiene and Environmental Health
Volume203
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Antibiotic resistance
  • Coral bleaching
  • Cytotoxicity
  • Enzymes
  • MTT-bioassay
  • Seawater aquarium
  • Vibrio alginolyticus
  • Virulence factor

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