Isolation of the minicircular DNA of Escherichia coli 15 as a DNA-Protein complex

Joachim Messing, Walter L. Staudenbauer, Peter Hans Hofschneider

Research output: Contribution to journalArticlepeer-review

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Abstract

1. 1. The minicircular DNA of Escherichia coli 15 can be isolated as a DNA-protein complex (form I{black star}) by sucrose gradient centrifugation in low salt (0.1 M NaCl). 2. 2. Form I{black star} can be converted into the non-complexed supercoiled form I in high salt (1 M NaCl) or by incubation with Pronase. It differs from the DNA-protein complexes of other plasmids by not undergoing a conversion to the open circular form II under conditions known to affect protein structure. 3. 3. Synthesis of the protein component of form I{black star} is inhibited by chloramphenicol. After a prolonged treatment with chloramphenicol the replication of the minicircular DNA continues but the newly replicated DNA is found almost exclusively in form I.

Original languageEnglish
Pages (from-to)465-471
Number of pages7
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume281
Issue number3
DOIs
StatePublished - 27 Oct 1972
Externally publishedYes

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