TY - JOUR
T1 - Isolation of the minicircular DNA of Escherichia coli 15 as a DNA-Protein complex
AU - Messing, Joachim
AU - Staudenbauer, Walter L.
AU - Hofschneider, Peter Hans
PY - 1972/10/27
Y1 - 1972/10/27
N2 - 1. 1. The minicircular DNA of Escherichia coli 15 can be isolated as a DNA-protein complex (form I{black star}) by sucrose gradient centrifugation in low salt (0.1 M NaCl). 2. 2. Form I{black star} can be converted into the non-complexed supercoiled form I in high salt (1 M NaCl) or by incubation with Pronase. It differs from the DNA-protein complexes of other plasmids by not undergoing a conversion to the open circular form II under conditions known to affect protein structure. 3. 3. Synthesis of the protein component of form I{black star} is inhibited by chloramphenicol. After a prolonged treatment with chloramphenicol the replication of the minicircular DNA continues but the newly replicated DNA is found almost exclusively in form I.
AB - 1. 1. The minicircular DNA of Escherichia coli 15 can be isolated as a DNA-protein complex (form I{black star}) by sucrose gradient centrifugation in low salt (0.1 M NaCl). 2. 2. Form I{black star} can be converted into the non-complexed supercoiled form I in high salt (1 M NaCl) or by incubation with Pronase. It differs from the DNA-protein complexes of other plasmids by not undergoing a conversion to the open circular form II under conditions known to affect protein structure. 3. 3. Synthesis of the protein component of form I{black star} is inhibited by chloramphenicol. After a prolonged treatment with chloramphenicol the replication of the minicircular DNA continues but the newly replicated DNA is found almost exclusively in form I.
UR - http://www.scopus.com/inward/record.url?scp=0015526194&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(72)90464-9
DO - 10.1016/0005-2787(72)90464-9
M3 - Article
C2 - 4565242
AN - SCOPUS:0015526194
SN - 0005-2787
VL - 281
SP - 465
EP - 471
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 3
ER -