Isolation and Taxonomic Characterization of a Halotolerant, Facultatively Iron-reducing Bacterium

Ramon A. Rossello-Mora, Frank Caccavo, Karin Osterlehner, Nina Springer, Stefan Spring, Dirk Schüler, Wolfgang Ludwig, Rudolf Amann, Marc Vanncanneyt, Karl Heinz Schleifer

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Abstract

The isolation of a halotolerant, Fe(III)-reducing, Gram-negative bacterium from surface sediments of the Sippewisset marsh in Woodshole (USA) will be described. Detailed molecular taxonomic studies such as comparative 16S rRNA sequence analysis and DNA-DNA hybridization experiments revealed that the newly isolated strain is closely related to the previously isolated strain BrY. Both strains belong to the DNA-DNA similarity group IV of Shewanella putrefaciens which has recently been described as Shewanella alga. Thus, the ability to reduce Fe(III) is no longer restricted to S. putrefaciens within the genus but is also found among strains of S. alga. A 16S rRNA targeted probe has been designed that enabled us to rapidly differentiate strains of S. alga from those of S. putrefaciens and other bacteria.

Original languageEnglish
Pages (from-to)569-573
Number of pages5
JournalSystematic and Applied Microbiology
Volume17
Issue number4
DOIs
StatePublished - 1995

Keywords

  • 16S rRNA
  • DNA-DNA hybridization
  • Iron reduction
  • Phylogeny
  • Shewanella sp.

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