Isolation and primary structure of the gene encoding fructose-1,6-bisphosphatase from Saccharomyces cerevisiae

Karl Dieter Entian, Rudi F. Vogel, Matthias Rose, Lucia Hofmann, Dieter Mecke

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The gene encoding Saccharomyces cerevisiae fructose-1,6-bisphosphatase (FBP1) was isolated. Constructed fbpi::HIS3 null mutants were unable to grow with ethanol, and growth was restored after transformation with the cloned fbp gene. The gene codes for a protein of 347 amino acid residues with an Mr of 38131. Homology with the pig kidney cortex and the sheep liver enzyme is 47.7% and 46.6%, respectively, within a central core of 328 amino acid residues. The cloned promoter size was 318 bp and allowed only low level expression of the gene. This indicates a positive activation site (UAS) upstream of the cloned DNA fragment.

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalFEBS Letters
Volume236
Issue number1
DOIs
StatePublished - 15 Aug 1988
Externally publishedYes

Keywords

  • (Saccharomyces cerevisiae)
  • Fructose-1,6-bisphosphatase
  • Gluconeogenesis
  • Nucleotide sequence
  • Null allele

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