Isolation and Electron Microscopic Analysis of Liver Cancer Cell Mitochondria

Yaschar Kabiri, Carola Eberhagen, Sabine Schmitt, Percy A. Knolle, Hans Zischka

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

Isolation of mitochondria is a crucial method for examining molecular details of this organelle’s manifold functions. Historically, mitochondrial isolations required large amounts of sample material which impeded their isolation from cultured cells. We have therefore developed a method allowing for controlled and reproducible isolation of intact and functional mitochondria from diverse cell types in culture. Here we provide a methodological update of this approach together with a protocol for the subsequent analysis of such isolated mitochondria by electron microscopy. Combining the isolation procedure with this powerful imaging method can reveal ultrastructural mitochondrial peculiarities in disease settings that might not be evident in intact cells and allows for assessment of mitochondrial membrane integrity and sample purity.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages277-287
Number of pages11
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2277
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Balch homogenizer
  • Cell culture
  • Electron microscopy
  • Image analysis
  • Mitochondria

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